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PMID: 2780312 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Detection and cloning of new HTLV-related endogenous sequences in man.

Nucleic acids research ·Vol. 17 ·No. 17 ·1989-09-12 ·Pages 6841-54

Perl A, Rosenblatt JD, Chen IS, DiVincenzo JP, Bever R, Poiesz BJ, Abraham GN

Abstract

Human T-cell leukemia virus (HTLV) type I-related endogenous sequences (HRES) have been cloned from a human genomic library. HRES-1/1 is present in DNA of all normal donors examined. By nucleotide sequence analysis, HRES-1/1 contains two potential open reading frames capable of encoding a p25 and a p15. A 684 bp flanking region 5' from the first ATG codon of p25 contains a TATA-box, a poly-adenylation signal, a putative tRNA primer binding site, and inverted repeats at locations which are typical of a retroviral long terminal repeat. Phylogenetic analysis suggests that HRES-1/1 entered the genome in primates, presumably as an exogenous retrovirus. From the deduced amino acid sequence of HRES-1/1 p25, residues 6-36 show a sequence homology of 32% and 39% to gag region segments of HTLV-I and HTLV-II, while residues 104-139 display a sequence homology of 33% and 28% to the gag regions of human immunodeficiency virus type 2 (HIV-2) and feline sarcoma virus (FSV), respectively. This suggests that the original exogenous virus infecting primate may be chimeric in structure. The HRES-1/1 genomic locus is transcriptionally active in lymphoid cells, melanoma cells, and embryonic tissues.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA, Viral/genetics,isolation & purification Genes, Viral Human T-lymphotropic virus 1/genetics Humans Molecular Sequence Data Phylogeny Primates/genetics,microbiology Retroviridae/genetics,isolation & purification Sequence Homology, Nucleic Acid Transcription, Genetic
Chemicals
DNA, Viral
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Perl A
Department of Medicine, University of Rochester School of Medicine, NY 14642.
Rosenblatt J D
Chen I S
DiVincenzo J P
Bever R
Poiesz B J
Abraham G N
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/17.17.6841
Published
1989-09-12
Pages
6841-54
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318416
Subset
IM
Grants
NIA NIH HHS · AG-06350 · United States
NIA NIH HHS · AG-08177 · United States
NIAID NIH HHS · AI-19658 · United States
External Links
PubMed source DOI: 10.1093/nar/17.17.6841 PMC Full Text View journal details
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