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PMID: 3839854 Published · ppublish English Journal Article

Characterization of human endogenous retroviral envelope RNA transcripts.

Journal of virology ·Vol. 56 ·No. 1 ·1985-10-00 ·Pages 176-82

Rabson AB, Hamagishi Y, Steele PE, Tykocinski M, Martin MA

Abstract

We characterized the structure of human endogenous retroviral env RNA transcripts by Northern blot hybridization and cDNA cloning. Polyadenylated 3.0- and 1.7-kilobase env RNAs can be identified in placenta, colon carcinoma, and breast carcinoma cells. We have obtained partial cDNA clones of both size classes of RNAs. Both env RNAs contained putative gp70 coding sequences; the 1.7-kilobase species, however, lacked sequences in the 3' env region which could specify a p15E analog. Both cDNA clones contained in-frame termination codons; thus, neither could encode full-length env proteins.

MeSH Terms
Amino Acid Sequence Base Sequence Cell Line Chromosome Mapping Cloning, Molecular DNA/genetics Female Humans Placenta/microbiology RNA, Messenger/genetics RNA, Viral/genetics Retroviridae/genetics Transcription, Genetic Viral Envelope Proteins/genetics
Chemicals
RNA, Messenger RNA, Viral Viral Envelope Proteins DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Rabson A B
Hamagishi Y
Steele P E
Tykocinski M
Martin M A
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.56.1.176-182.1985
Published
1985-10-00
Pages
176-82
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC252503
Subset
IM
Databases
GENBANK
M11119
External Links
PubMed source DOI: 10.1128/JVI.56.1.176-182.1985 PMC Full Text View journal details
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