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PMID: 6310547 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A genetic locus regulates the expression of tissue-specific mRNAs from multiple transcription units.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 19 ·1982-10-00 ·Pages 5823-7

Levy DE, Lerner RA, Wilson MC

Abstract

129 GIX- mice, unlike animals of the congeneic partner strain GIX+, do not express significant amounts of the retroviral antigens gp70 and p30. Evidence is presented indicating that the GIX phenotype is specified by a distinct regulatory gene acting on multiple transcription units to control the levels of accumulation of specific mRNA species. The steady-state levels of retroviral-homologous mRNA from the tissues of GIX+ and GIX- mice were examined by blot hybridization using as probes DNA fragments from cloned murine leukemia viruses. RNA potentially encoding viral antigens was reduced or absent in GIX- mice, even though no differences in integrated viral genomes were detected between these congeneic strains by DNA blotting. Tissue-specific patterns of accumulation of these RNA species were detected in brain, epididymis, liver, spleen, and thymus, and several distinct RNA species were found to be coordinately regulated with the GIX phenotype. Measurements of RNA synthesis suggest a major role for transcriptional control in the regulation of some retroviral messages.

MeSH Terms
Animals Base Sequence Brain/metabolism Cell Nucleus/metabolism Chromosomes/physiology Cloning, Molecular DNA/genetics DNA, Recombinant/metabolism Genes Leukemia Virus, Murine/genetics Liver/metabolism Male Mice Mice, Mutant Strains Nucleic Acid Hybridization RNA, Messenger/genetics Spermatozoa/metabolism Thymus Gland/metabolism Transcription, Genetic
Chemicals
DNA, Recombinant RNA, Messenger DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Levy D E
Lerner R A
Wilson M C
References (45)
45 references, click to expand
  1. Nucleotide sequences associated with differences in electrophoretic mobility of envelope glycoprotein gp70 and with GIX antigen phenotype of certain murine leukemia viruses.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1642-5 PMID: 6154937
  2. Molecular cloning of infectious integrated murine leukemia virus DNA from infected mouse cells.
    Proc Natl Acad Sci U S A. 1980 Jan;77(1):614-8 PMID: 6244569
  3. Molecular cloning of unintegrated and a portion of integrated moloney murine leukemia viral DNA in bacteriophage lambda.
    J Virol. 1980 Oct;36(1):254-63 PMID: 6255210
  4. Structure of endogenous murine leukemia virus DNA in mouse genomes.
    Proc Natl Acad Sci U S A. 1980 Oct;77(10):5774-8 PMID: 6255464
  5. Identification of ecotropic proviral sequences in inbred mouse strains with a cloned subgenomic DNA fragment.
    Proc Natl Acad Sci U S A. 1980 Oct;77(10):5779-83 PMID: 6255465
  6. Relationship of GIX antigen expression to the glycosylation of murine leukemia virus glycoprotein.
    Proc Natl Acad Sci U S A. 1980 Nov;77(11):6420-4 PMID: 6935656
  7. Regulation of adenovirus-2 gene expression at the level of transcriptional termination and RNA processing.
    Nature. 1981 Mar 12;290(5802):113-8 PMID: 7207591
  8. Variety in the mechanisms of adenovirus gene control.
    Ann N Y Acad Sci. 1980;354:466-71 PMID: 6939405
  9. A cell surface antigen of the mouse related to xenotropic MuLv defined by naturally occurring antibody and monoclonal antibody. Relation to Gix G(rada1), G(aksl2) systems of MuLV-related antigens.
    J Exp Med. 1981 Sep 1;154(3):659-75 PMID: 6268731
  10. Lipopolysaccharide induces retroviral antigen expression in 129/J mouse lymphocytes: evidence for assembly of a defective viral particle.
    J Virol. 1981 Mar;37(3):1044-50 PMID: 6164797
  11. Transcriptional control in the production of liver-specific mRNAs.
    Cell. 1981 Mar;23(3):731-9 PMID: 7226226
  12. Nucleotide sequence of Moloney murine leukaemia virus.
    Nature. 1981 Oct 15-21;293(5833):543-8 PMID: 6169994
  13. Control of messenger RNA concentration by differential cytoplasmic half-life. Adenovirus messenger RNAs from transcription units 1A and 1B.
    J Mol Biol. 1981 May 25;148(3):231-51 PMID: 7299816
  14. Specific antigens on methylcholanthrene-induced tumors of mice.
    Transplant Proc. 1981 Dec;13(4):1759-61 PMID: 6173943
  15. Heritable differences among gp70-like molecules on C3H ultraviolet light-induced fibrosarcomas.
    Transplant Proc. 1981 Dec;13(4):1782-6 PMID: 6277056
  16. The same genetic locus directs differentiation-linked expression of endogenous retrovirus gp70 on thymocytes and spleen cells in the mouse.
    Immunogenetics. 1982 Jan;15(1):103-8 PMID: 6282741
  17. Cloning and characterization of an envelope-specific probe from xenotropic murine leukemia proviral DNA.
    J Virol. 1982 Jan;41(1):228-36 PMID: 6283115
  18. Transcriptional regulation of hemoglobin switching in chicken embryos.
    Mol Cell Biol. 1981 Mar;1(3):281-8 PMID: 6086008
  19. Specific inhibition of nuclear RNA polymerase II by alpha-amanitin.
    Science. 1970 Oct 23;170(3956):447-9 PMID: 4918258
  20. Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event.
    Biochemistry. 1970 Oct 27;9(22):4428-40 PMID: 4919547
  21. The G-IX system. A cell surface allo-antigen associated with murine leukemia virus; implications regarding chromosomal integration of the viral genome.
    J Exp Med. 1971 Jun 1;133(6):1334-55 PMID: 5576334
  22. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 PMID: 4504350
  23. Host control of endogenous murine leukemia virus gene expression: concentrations of viral proteins in high and low leukemia mouse strains.
    Proc Natl Acad Sci U S A. 1974 Sep;71(9):3682-6 PMID: 4372631
  24. The oncornavirus glycoprotein gp69/71: a constituent of the surface of normal and malignant thymocytes.
    J Exp Med. 1975 Jan 1;141(1):172-87 PMID: 46909
  25. Relation of GIX antigen of thymocytes to envelope glycoprotein of murine leukemia virus.
    J Exp Med. 1975 Jan 1;141(1):188-97 PMID: 46910
  26. Biochemical evidence linking the GIX thymocyte surface antigen to the gp69/71 envelope glycoprotein of murine leukemia virus.
    J Exp Med. 1975 Jan 1;141(1):198-205 PMID: 163885
  27. New mutant and congenic mouse stocks expressing the murine leukemia virus-associated thymocyte surface antigen GIX.
    J Exp Med. 1975 Aug 1;142(2):512-7 PMID: 167097
  28. Expression of murine leukemia virus envelope glycoprotein gp69/71 on mouse thymocytes. Evidence for two structural variants distinguished by presence vs. absence of GIX antigen.
    J Exp Med. 1975 Aug 1;142(2):518-23 PMID: 167098
  29. Endogenous oncornaviral gene expression in adult and fetal mice: quantitative, histologic, and physiologic studies of the major viral glycorprotein, gp70.
    J Exp Med. 1976 Jan 1;143(1):151-66 PMID: 172586
  30. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  31. Relationship between the oncornavirus gene product gp70 and a major protein secretion of the mouse genital tract.
    Nature. 1976 Feb 12;259(5543):497-9 PMID: 56712
  32. Heredity of the GIX thymocyte antigen associated with murine leukemia virus: segregation data simulating genetic linkage.
    Proc Natl Acad Sci U S A. 1976 Jun;73(6):2077-81 PMID: 180539
  33. Polymorphism of the major envelope glycoprotein (gp70) of murine C-type viruses: virion associated and differentiation antigens encoded by a multi-gene family.
    Nature. 1977 May 5;267(5606):23-8 PMID: 193039
  34. Expression of AKR murine leukemia virus gp71-like and BALB(X) gp-71-like antigens in normal mouse tissues in the absence of overt virus expression.
    J Exp Med. 1977 Aug 1;146(2):422-34 PMID: 195002
  35. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  36. The initiation sites for RNA transcription in Ad2 DNA.
    Cell. 1977 Nov;12(3):733-9 PMID: 922890
  37. Xenotropic type C viruses.
    Curr Top Microbiol Immunol. 1978;79:111-213 PMID: 77206
  38. Large T1 oligonucleotides of Moloney leukemia virus missing in an env gene recombinant, HIX, are present on an intracellular 21S Moloney viral RNA species.
    Proc Natl Acad Sci U S A. 1978 Jun;75(6):2964-8 PMID: 96445
  39. Leukemia virus genomes in the chromosomal DNA of the mouse.
    Harvey Lect. 1978;71:173-92 PMID: 213400
  40. The nature of the association between the murine leukemia virus envelope proteins.
    Virology. 1978 Dec;91(2):345-51 PMID: 217151
  41. Transcriptional regulation of the ovalbumin and conalbumin genes by steroid hormones in chick oviduct.
    J Biol Chem. 1979 Sep 25;254(18):9050-8 PMID: 479179
  42. Production of mRNA in Chinese hamster cells: relationship of the rate of synthesis to the cytoplasmic concentration of nine specific mRNA sequences.
    Cell. 1979 Aug;17(4):1025-35 PMID: 487428
  43. Identification of procollagen mRNAs transferred to diazobenzyloxymethyl paper from formaldehyde agarose gels.
    Nucleic Acids Res. 1979 Aug 10;6(11):3559-67 PMID: 573889
  44. Rate of synthesis and concentration of specific mRNA sequences in cultured Chinese hamster ovary cells compared to liver cells.
    Biochem Biophys Res Commun. 1980 Jan 29;92(2):485-91 PMID: 7356477
  45. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.19.5823
Published
1982-10-00
Pages
5823-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC347002
Subset
IM
Grants
NCI NIH HHS · CA 27489 · United States
NIGMS NIH HHS · GM-07616 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.19.5823 PMC Full Text View journal details
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