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PMID: 2770691 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The linear mitochondrial plasmid pClK1 of the phytopathogenic fungus Claviceps purpurea may code for a DNA polymerase and an RNA polymerase.

Molecular & general genetics : MGG ·Vol. 217 ·No. 1 ·1989-05-00 ·Pages 132-40

Oeser B, Tudzynski P

Abstract

Plasmid pClK1, a linear mitochondrial plasmid of Claviceps purpurea, was completely sequenced. The sequence contains two long open reading frames (ORF1, 3291 bp; ORF2, 2910 bp), and at least four smaller ORFs. The potential polypeptide derived from ORF1 shows homology to the family B type DNA polymerases. The product of ORF2 has significant homology to the mitochondrial RNA polymerase of yeast and RNA polymerases from bacteriophages. ORF1 and ORF2 show homology to URF3 and URF1 of the maize plasmids S1 and S2, respectively. No homology to any published protein sequence was found for the smaller ORFs. The origin of the terminal protein attached to the 5' ends of pClK1 remains open; several alternatives for its origin are discussed. The sequence data as a whole confirm the virus-like character of pClK1 already postulated from structural properties. Thus pClK1 together with S plasmids of maize and several other linear plasmids make up a distinct class of DNA species of plants and fungi probably derived from a common virus-like ancestor.

MeSH Terms
Amino Acid Sequence Base Sequence Blotting, Southern Claviceps/enzymology,genetics Cloning, Molecular DNA, Fungal/genetics DNA, Mitochondrial/genetics DNA-Directed DNA Polymerase/genetics DNA-Directed RNA Polymerases/genetics Electrophoresis, Polyacrylamide Gel Models, Genetic Molecular Sequence Data Plasmids Sequence Homology, Nucleic Acid
Chemicals
DNA, Fungal DNA, Mitochondrial DNA-Directed RNA Polymerases DNA-Directed DNA Polymerase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Oeser B
Allgemeine Botanik/Mikrobiologie, Botanisches Institut, Westfälische Wilhelms-Universität, Münster, Federal Republic of Germany.
Tudzynski P
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00330952
Published
1989-05-00
Pages
132-40
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00330952
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