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PMID: 2762147 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation of human cDNA clones of ski and the ski-related gene, sno.

Nucleic acids research ·Vol. 17 ·No. 14 ·1989-07-25 ·Pages 5489-500

Nomura N, Sasamoto S, Ishii S, Date T, Matsui M, Ishizaki R

Abstract

cDNA clones of ski and the ski-related gene, sno, were obtained by screening human cDNA libraries. The predicted open reading frame of h-ski could encode a protein of 728 amino acid residues. The h-ski protein is highly homologous with the v-ski protein. The overall homology between h-ski and v-ski is 91% at the amino acid level. DNA sequencing analysis revealed two types of cDNA clones from the sno (ski-related novel gene) gene, possibly due to alternative splicing. The first type, named snoN (non Alu-containing), encoded a protein of 684 amino acid residues. The second type, named snoA (Alu-containing), encoded a protein of 415 amino acid residues. The first 366 amino acid residues of snoN and snoA are the same, but subsequent amino acids show divergence. Several transcripts of h-ski (6.0, 4.7, 3.8, 3.0, 2.1 and 1.8 kb) were detected. The mRNAs of h-sno were 6.2, 4.4 and 3.2kb.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Biological Evolution Cell Line DNA/genetics,isolation & purification DNA, Neoplasm/genetics,isolation & purification DNA-Binding Proteins Humans Intracellular Signaling Peptides and Proteins Molecular Sequence Data Multigene Family Oncogenes Proteins/genetics Proto-Oncogene Proteins Sequence Homology, Nucleic Acid Software
Chemicals
DNA, Neoplasm DNA-Binding Proteins Intracellular Signaling Peptides and Proteins Proteins Proto-Oncogene Proteins SKIL protein, human SKI protein, human DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Nomura N
Molecular Oncology Laboratory, Nippon Veterinary and Zootechnical College, Tokyo, Japan.
Sasamoto S
Ishii S
Date T
Matsui M
Ishizaki R
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/17.14.5489
Published
1989-07-25
Pages
5489-500
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318172
Subset
IM
Databases
GENBANK
X15217, X15218, X15219
External Links
PubMed source DOI: 10.1093/nar/17.14.5489 PMC Full Text View journal details
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