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PMID: 2738026 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Lipopolysaccharide mutants of Rhizobium meliloti are not defective in symbiosis.

Journal of bacteriology ·Vol. 171 ·No. 7 ·1989-07-00 ·Pages 3961-7

Clover RH, Kieber J, Signer ER

Abstract

Mutants of Rhizobium meliloti selected primarily for bacteriophage resistance fall into 13 groups. Mutants in the four best-characterized groups (class A, lpsB, lpsC, and class D), which map to the rhizobial chromosome, appear to affect lipopolysaccharide (LPS) as judged by the reactivity with monoclonal antibodies and behavior on sodium dodecyl sulfate-polyacrylamide gels of extracted LPS. Mutations in all 13 groups, in an otherwise wild-type genetic background, are Fix+ on alfalfa. This suggests that LPS does not play a major role in symbiosis. Mutations in lpsB, however, are Fix- in one particular genetic background, evidently because of the cumulative effect of several independent background mutations. In addition, an auxotrophic mutation evidently equivalent to Escherichia coli carAB is Fix- on alfalfa.

MeSH Terms
Electrophoresis, Polyacrylamide Gel Genes, Bacterial Lipopolysaccharides/genetics,isolation & purification,physiology Mutation Phenotype Plasmids Rhizobium/genetics,isolation & purification Sodium Dodecyl Sulfate Symbiosis
Chemicals
Lipopolysaccharides Sodium Dodecyl Sulfate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Clover R H
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
Kieber J
Signer E R
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.7.3961-3967.1989
Published
1989-07-00
Pages
3961-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC210148
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.171.7.3961-3967.1989 PMC Full Text View journal details
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