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PMID: 2714251 Published · ppublish English Journal Article

Asymmetry and polarity of nucleosomes in chicken erythrocyte chromatin.

The EMBO journal ·Vol. 8 ·No. 1 ·1989-01-00 ·Pages 229-38

Satchwell SC, Travers AA

Abstract

Nucleosome dimers containing, on average, a single molecule of histone H5 have been isolated from chicken erythrocyte nuclei and the associated DNA fragments cloned and sequenced. The average sequence organization of at least one of the two nucleosomes in the dimers is highly asymmetric and suggests that the torsional, as well as the axial, flexibility of DNA is a determinant of nucleosome positioning. On average the nucleosome dimer is a polar structure containing linker DNA of variable lengths. The sequences associated with H5 containing nucleosomes and core particles are sufficiently different to indicate that removal of histone H5 (or H1) from chromatin may result in the migration of the histone octamer and a consequent exposure of sites for regulatory proteins.

MeSH Terms
Animals Base Sequence Chickens/blood Chromatin/ultrastructure DNA/analysis Erythrocytes/ultrastructure Histones/analysis Nucleic Acid Conformation Nucleosomes/ultrastructure Regulatory Sequences, Nucleic Acid
Chemicals
Chromatin Histones Nucleosomes DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Satchwell S C
MRC Laboratory of Molecular Biology, Cambridge, UK.
Travers A A
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-01-00
Pages
229-38
Language
English
Region
England
NLM ID
8208664
PMCID
PMC400794
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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