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PMID: 3671072 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Chromatin structures: dissecting their mixed patterns in nuclease digests.

Nucleic acids research ·Vol. 15 ·No. 19 ·1987-10-12 ·Pages 8087-103

Drinkwater RD, Wilson PJ, Skinner JD, Burgoyne LA

Abstract

Four separate features could be distinguished in Fe-DNAase-1 digestions of human lymphoblast nuclei: a di-nucleosomal (2N) repeat, a mono-nucleosomal (1N) repeat, a component of "random" DNA, and triple splitting of major peaks. The random component is major, is unlikely to be completely artifactual, and is what would be expected from the face to face layering model of Subirana et. al., (1). The 2N pattern appeared to be associated with compact, metaphase-type chromatin, whereas the 1N pattern was associated with more exposed chromatin. These two modes are explained in terms of orderly back-to-back folding of zig-zag nucleofilaments, and face-to-face folding respectively. Hybridization studies indicated that the centromeric classes of repetitive DNA had the same digestion spectra as the major interspersed classes of repetitive DNA, and DNA enriched in transcriptionally active sequences. It is suggested that current coil models are all inadequate explanations of higher order chromatin packing.

MeSH Terms
Chromatin/metabolism,ultrastructure Deoxyribonuclease I Humans Lymphocytes/ultrastructure Mitosis Models, Biological Repetitive Sequences, Nucleic Acid Transcription, Genetic
Chemicals
Chromatin Deoxyribonuclease I
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Drinkwater R D
Flinders Medical Centre, Adelaide, SA, Australia.
Wilson P J
Skinner J D
Burgoyne L A
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43 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.19.8087
Published
1987-10-12
Pages
8087-103
Language
English
Region
England
NLM ID
0411011
PMCID
PMC306328
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/15.19.8087 PMC Full Text View journal details
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