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PMID: 2685559 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A cis-acting element in the promoter region of the murine c-myc gene is necessary for transcriptional block.

Molecular and cellular biology ·Vol. 9 ·No. 12 ·1989-12-00 ·Pages 5340-9

Miller H, Asselin C, Dufort D, Yang JQ, Gupta K, Marcu KB, Nepveu A

Abstract

A block to elongation of transcription has been shown to occur within the first exon of the human and murine c-myc genes. The extent of this block was found to vary with the physiological state of cells, indicating that modulation of the transcriptional block can serve to control the expression of this gene. To determine which sequences are required in cis for the transcriptional block, we generated a series of constructs containing various portions of murine c-myc 5'-flanking and exon 1 sequences. We established populations of HeLa and CV-1 cells stably transfected with these constructs. The transcription start sites were determined by S1 nuclease mapping analysis, and the extent of transcriptional block was measured by nuclear run-on transcription assays. Our results demonstrate that at least two cis-acting elements are necessary for the transcriptional block. A 3' element was found to be located in the region where transcription stopped and showed features reminiscent of some termination sites found in procaryotes. A 5' element was positioned between the P1 and P2 (C. Asselin, A. Nepveu, and K. B. Marcu, Oncogene 4:549-558, 1989). Removal of the more 3' binding site abolished the transcriptional block.

MeSH Terms
Animals Cell Line Cell Nucleus/metabolism Exons Gene Expression Regulation, Neoplastic HeLa Cells/metabolism Humans Mice Plasmids Promoter Regions, Genetic Protein-Tyrosine Kinases/genetics Proto-Oncogene Proteins/genetics Proto-Oncogene Proteins c-myc Proto-Oncogenes Restriction Mapping Transcription, Genetic Transfection
Chemicals
Proto-Oncogene Proteins Proto-Oncogene Proteins c-myc Protein-Tyrosine Kinases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Miller H
Ludwig Institute for Cancer Research, Montreal Branch, Canada.
Asselin C
Dufort D
Yang J Q
Gupta K
Marcu K B
Nepveu A
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.12.5340-5349.1989
Published
1989-12-00
Pages
5340-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363702
Subset
IM
Grants
NCI NIH HHS · CA36246 · United States
External Links
PubMed source DOI: 10.1128/mcb.9.12.5340-5349.1989 PMC Full Text View journal details
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