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PMID: 2684645 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Proliferation of human malignant melanomas is inhibited by antisense oligodeoxynucleotides targeted against basic fibroblast growth factor.

The EMBO journal ·Vol. 8 ·No. 12 ·1989-12-01 ·Pages 3685-91

Becker D, Meier CB, Herlyn M

Abstract

Human malignant melanomas, unlike normal melanocytes, can proliferate in the absence of exogenous basic fibroblast growth factor (bFGF). Exposure of primary melanomas in the vertical growth phase and metastatic melanomas to antisense oligodeoxynucleotides targeted against three different sites of human bFGF mRNA inhibited cell proliferation and colony formation in soft-agar. In contrast, exposure of human bFGF sense or antisense oligonucleotides complementary to human beta-nerve growth factor or insulin-like growth factor I mRNA had no such effects. These experiments indicate that activation of the bFGF gene may play an important role in the progression from melanocytic precursor lesions to malignant melanoma.

MeSH Terms
Antineoplastic Agents/pharmacology Base Sequence Cell Division/drug effects Cell Line Colony-Forming Units Assay Fibroblast Growth Factors/antagonists & inhibitors,genetics Growth Inhibitors/pharmacology Humans Kinetics Melanoma/genetics,metabolism,pathology Molecular Sequence Data Oligonucleotides/metabolism,pharmacology Oligonucleotides, Antisense Tumor Cells, Cultured
Chemicals
Antineoplastic Agents Growth Inhibitors Oligonucleotides Oligonucleotides, Antisense Fibroblast Growth Factors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Becker D
Department of Tumor Biology, M.D. Anderson Cancer Center, Houston, TX 77030.
Meier C B
Herlyn M
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37 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-12-01
Pages
3685-91
Language
English
Region
England
NLM ID
8208664
PMCID
PMC402051
Subset
IM
Grants
NCI NIH HHS · CA16672 · United States
NCI NIH HHS · CA25874 · United States
External Links
PubMed source PMC Full Text View journal details
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