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PMID: 2839841 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Induction of nerve growth factor receptors on cultured human melanocytes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 14 ·1988-07-00 ·Pages 5282-6

Peacocke M, Yaar M, Mansur CP, Chao MV, Gilchrest BA

Abstract

Normal differentiation and malignant transformation of human melanocytes involve a complex series of interactions during which both genetic and environmental factors play roles. At present, the regulation of these processes is poorly understood. We have induced the expression of nerve growth factor (NGF) receptors on cultured human melanocytes with phorbol 12-tetradecanoate 13-acetate and have correlated this event with the appearance of a more differentiated, dendritic morphology. Criteria for NGF receptor expression included protein accumulation and cell-surface immunofluorescent staining with a monoclonal antibody directed against the human receptor and induction of the messenger RNA species as determined by blot-hybridization studies. The presence of the receptor could also be induced by UV irradiation or growth factor deprivation. The NGF receptor is inducible in cultured human melanocytes, and we suggest that NGF may modulate the behavior of this neural crest-derived cell in the skin.

MeSH Terms
Cell Line Electrophoresis, Polyacrylamide Gel Fluorescent Antibody Technique Humans Immunoassay Melanocytes/cytology,drug effects,metabolism Nucleic Acid Hybridization RNA, Messenger/biosynthesis Receptors, Cell Surface/biosynthesis,drug effects,genetics Receptors, Nerve Growth Factor Tetradecanoylphorbol Acetate/pharmacology
Chemicals
RNA, Messenger Receptors, Cell Surface Receptors, Nerve Growth Factor Tetradecanoylphorbol Acetate
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Peacocke M
U.S. Department of Agriculture Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111.
Yaar M
Mansur C P
Chao M V
Gilchrest B A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.14.5282
Published
1988-07-00
Pages
5282-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC281734
Subset
IM
Grants
NIA NIH HHS · AG00354 · United States
NCI NIH HHS · CA45687 · United States
NINDS NIH HHS · NS21072 · United States
External Links
PubMed source DOI: 10.1073/pnas.85.14.5282 PMC Full Text View journal details
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