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PMID: 2682633 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cloning a eukaryotic DNA glycosylase repair gene by the suppression of a DNA repair defect in Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 20 ·1989-10-00 ·Pages 7961-5

Chen J, Derfler B, Maskati A, Samson L

Abstract

If eukaryotic genes could protect bacteria with defects in DNA repair, this effect could be exploited for the isolation of eukaryotic DNA repair genes. We have thus cloned a DNA repair gene from Saccharomyces cerevisiae that directs the synthesis of a DNA glycosylase that specifically releases 3-methyladenine from alkylated DNA and in so doing protects alkylation-sensitive Escherichia coli from killing by methylating agents. The cloned yeast gene was then used to generate a mutant strain of S. cerevisiae that carries a defect in the glycosylase gene and is extremely sensitive to DNA methylation. This approach may allow the isolation of a large number of eukaryotic DNA repair genes.

MeSH Terms

Blotting, Northern Blotting, Southern Cloning, Molecular/methods DNA Damage DNA Glycosylases DNA Repair Drug Resistance Escherichia coli/enzymology,genetics,growth & development Genes, Fungal Methyl Methanesulfonate/pharmacology N-Glycosyl Hydrolases/genetics,metabolism Recombinant Proteins/metabolism Restriction Mapping Saccharomyces cerevisiae/drug effects,enzymology,genetics

Chemicals

Recombinant Proteins Methyl Methanesulfonate 3-methyladenine-DNA glycosylase DNA Glycosylases N-Glycosyl Hydrolases

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Chen J

Harvard School of Public Health, Charles A. Dana Laboratory of Toxicology, Boston, MA 02115.

Derfler B

Maskati A

Samson L

References (23)

23 references, click to expand

Cloning of human purine-nucleoside phosphorylase cDNA sequences by complementation in Escherichia coli.
Proc Natl Acad Sci U S A. 1983 Jul;80(14):4281-5 PMID: 6410388
Adaptation to alkylation resistance involves the induction of a DNA glycosylase.
Nature. 1982 Apr 22;296(5859):773-5 PMID: 7040984
Cloning of Escherichia coli genes encoding 3-methyladenine DNA glycosylases I and II.
Mol Gen Genet. 1984;197(3):368-72 PMID: 6098799
Identification of functional murine adenosine deaminase cDNA clones by complementation in Escherichia coli.
J Biol Chem. 1985 Aug 25;260(18):10299-307 PMID: 2410423
Genetic complementation of UV-induced DNA repair in Chinese hamster ovary cells by the denV gene of phage T4.
Proc Natl Acad Sci U S A. 1985 Nov;82(22):7656-60 PMID: 3865186
Escherichia coli gene induction by alkylation treatment.
Genetics. 1986 Jan;112(1):11-26 PMID: 3080354
The isolation and characterization of an alkylating-agent-sensitive yeast mutant, ngs1.
Mutat Res. 1986 May;165(3):129-37 PMID: 3517634
Suppression of human DNA alkylation-repair defects by Escherichia coli DNA-repair genes.
Proc Natl Acad Sci U S A. 1986 Aug;83(15):5607-10 PMID: 3526337
Reduction of the toxicity and mutagenicity of alkylating agents in mammalian cells harboring the Escherichia coli alkyltransferase gene.
Proc Natl Acad Sci U S A. 1986 Sep;83(17):6292-6 PMID: 3529080
DNA alkylation repair and the induction of cell death and sister chromatid exchange in human cells.
Carcinogenesis. 1987 Feb;8(2):227-30 PMID: 3802405
Complementation of sensitivity to alkylating agents in Escherichia coli and Chinese hamster ovary cells by expression of a cloned bacterial DNA repair gene.
EMBO J. 1986 Dec 1;5(12):3195-200 PMID: 3545815
A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains.
Genetics. 1987 Aug;116(4):541-5 PMID: 3305158
Deoxyribonucleic acid repair in the yeast Saccharomyces cerevisiae.
Microbiol Rev. 1988 Mar;52(1):70-102 PMID: 3280967
A second DNA methyltransferase repair enzyme in Escherichia coli.
Proc Natl Acad Sci U S A. 1988 May;85(9):3039-43 PMID: 3283737
The Saccharomyces cerevisiae RAD18 gene encodes a protein that contains potential zinc finger domains for nucleic acid binding and a putative nucleotide binding sequence.
Nucleic Acids Res. 1988 Jul 25;16(14B):7119-31 PMID: 2970061
Regulation and expression of the adaptive response to alkylating agents.
Annu Rev Biochem. 1988;57:133-57 PMID: 3052269
Isolation and characterization of MMS-sensitive mutants of Saccharomyces cerevisiae.
Genetics. 1977 May;86(1):33-55 PMID: 195865
Production of a functional eukaryotic enzyme in Escherichia coli: cloning and expression of the yeast structural gene for imidazole-glycerolphosphate dehydratase (his3).
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5255-9 PMID: 341150
Isolation of genes by complementation in yeast: molecular cloning of a cell-cycle gene.
Proc Natl Acad Sci U S A. 1980 Apr;77(4):2119-23 PMID: 6246523
Escherichia coli mutants deficient in 3-methyladenine-DNA glycosylase.
J Mol Biol. 1980 Jun 15;140(1):101-27 PMID: 6997501
Two differentially regulated mRNAs with different 5' ends encode secreted with intracellular forms of yeast invertase.
Cell. 1982 Jan;28(1):145-54 PMID: 7039847
Induction of a DNA glycosylase for N-methylated purines is part of the adaptive response to alkylating agents.
Nature. 1982 Apr 22;296(5859):770-3 PMID: 7040983
Eviction and transplacement of mutant genes in yeast.
Methods Enzymol. 1983;101:211-28 PMID: 6310325

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.86.20.7961

Published

1989-10-00

Pages

7961-5

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC298192

Subset

Grants

NIEHS NIH HHS · 1-P0-ES03926 · United States

External Links

PubMed source DOI: 10.1073/pnas.86.20.7961 PMC Full Text View journal details

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Cloning a eukaryotic DNA glycosylase repair gene by the suppression of a DNA repair defect in Escherichia coli.

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