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PMID: 2661535 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Similarity of Escherichia coli propanediol oxidoreductase (fucO product) and an unusual alcohol dehydrogenase from Zymomonas mobilis and Saccharomyces cerevisiae.

Journal of bacteriology ·Vol. 171 ·No. 7 ·1989-07-00 ·Pages 3754-9

Conway T, Ingram LO

Abstract

The gene that encodes 1,2-propanediol oxidoreductase (fucO) from Escherichia coli was sequenced. The reading frame specified a protein of 383 amino acids (including the N-terminal methionine), with an aggregate molecular weight of 40,642. The induction of fucO transcription, which occurred in the presence of fucose, was confirmed by Northern blot analysis. In E. coli, the primary fucO transcript was approximately 2.1 kilobases in length. The 5' end of the transcript began more than 0.7 kilobase upstream of the fucO start codon within or beyond the fucA gene. Propanediol oxidoreductase exhibited 41.7% identity with the iron-containing alcohol dehydrogenase II from Zymomonas mobilis and 39.5% identity with ADH4 from Saccharomyces cerevisiae. These three proteins did not share homology with either short-chain or long-chain zinc-containing alcohol dehydrogenase enzymes. We propose that these three unusual alcohol dehydrogenases define a new family of enzymes.

MeSH Terms
Alcohol Oxidoreductases/genetics,isolation & purification Amino Acid Sequence Bacterial Proteins/genetics,isolation & purification Base Sequence Cloning, Molecular Escherichia coli/enzymology,genetics Fungal Proteins/genetics,isolation & purification Genes, Bacterial Genes, Fungal Gram-Negative Anaerobic Bacteria/enzymology,genetics Molecular Sequence Data Saccharomyces cerevisiae/enzymology,genetics Sequence Homology, Nucleic Acid Transcription, Genetic
Chemicals
Bacterial Proteins Fungal Proteins Alcohol Oxidoreductases lactaldehyde reductase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Conway T
School of Biological Sciences, University of Nebraska, Lincoln 68588-0118.
Ingram L O
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.7.3754-3759.1989
Published
1989-07-00
Pages
3754-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC210121
Subset
IM
Databases
GENBANK
M27177
External Links
PubMed source DOI: 10.1128/jb.171.7.3754-3759.1989 PMC Full Text View journal details
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