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PMID: 26163575 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Aquaporins Contribute to ABA-Triggered Stomatal Closure through OST1-Mediated Phosphorylation.

The Plant cell ·Vol. 27 ·No. 7 ·2015-07-00 ·Pages 1945-54

Grondin A, Rodrigues O, Verdoucq L, Merlot S, Leonhardt N, Maurel C

Abstract

Stomatal movements in response to environmental stimuli critically control the plant water status. Although these movements are governed by osmotically driven changes in guard cell volume, the role of membrane water channels (aquaporins) has remained hypothetical. Assays in epidermal peels showed that knockout Arabidopsis thaliana plants lacking the Plasma membrane Intrinsic Protein 2;1 (PIP2;1) aquaporin have a defect in stomatal closure, specifically in response to abscisic acid (ABA). ABA induced a 2-fold increase in osmotic water permeability (Pf) of guard cell protoplasts and an accumulation of reactive oxygen species in guard cells, which were both abrogated in pip2;1 plants. Open stomata 1 (OST1)/Snf1-related protein kinase 2.6 (SnRK2.6), a protein kinase involved in guard cell ABA signaling, was able to phosphorylate a cytosolic PIP2;1 peptide at Ser-121. OST1 enhanced PIP2;1 water transport activity when coexpressed in Xenopus laevis oocytes. Upon expression in pip2;1 plants, a phosphomimetic form (Ser121Asp) but not a phosphodeficient form (Ser121Ala) of PIP2;1 constitutively enhanced the Pf of guard cell protoplasts while suppressing its ABA-dependent activation and was able to restore ABA-dependent stomatal closure in pip2;1. This work supports a model whereby ABA-triggered stomatal closure requires an increase in guard cell permeability to water and possibly hydrogen peroxide, through OST1-dependent phosphorylation of PIP2;1 at Ser-121.

MeSH Terms
Abscisic Acid/pharmacology Animals Aquaporins/metabolism Arabidopsis Proteins/metabolism Cell Membrane Permeability/drug effects Enzyme Activation/drug effects Genetic Complementation Test Movement/drug effects Mutation/genetics Oocytes/drug effects,metabolism Phosphorylation/drug effects Phosphoserine/metabolism Plant Stomata/cytology,drug effects,physiology Protein Kinases/metabolism Protoplasts/drug effects,metabolism Reactive Oxygen Species/metabolism Xenopus
Chemicals
Aquaporins Arabidopsis Proteins Reactive Oxygen Species plasma membrane intrinsic protein 2 Arabidopsis Phosphoserine Abscisic Acid Protein Kinases OST1 protein, Arabidopsis
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Grondin Alexandre ORCID
Biochimie et Physiologie Moléculaire des Plantes, Unité Mixte de Recherche 5004, CNRS/INRA/Montpellier SupAgro/Université Montpellier, F-34060 Montpellier, Cedex 2, France.
Rodrigues Olivier
Biochimie et Physiologie Moléculaire des Plantes, Unité Mixte de Recherche 5004, CNRS/INRA/Montpellier SupAgro/Université Montpellier, F-34060 Montpellier, Cedex 2, France.
Verdoucq Lionel
Biochimie et Physiologie Moléculaire des Plantes, Unité Mixte de Recherche 5004, CNRS/INRA/Montpellier SupAgro/Université Montpellier, F-34060 Montpellier, Cedex 2, France.
Merlot Sylvain
Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Sciences Plant Saclay, F-91198 Gif sur Yvette Cedex, France.
Leonhardt Nathalie ORCID
Laboratoire de Biologie du Développement des Plantes, CEA Cadarache, Unité Mixte de Recherche 7265, CNRS/CEA/Aix-Marseille Université, F-13108 Saint-Paul-lez-Durance, France.
Maurel Christophe ORCID
Biochimie et Physiologie Moléculaire des Plantes, Unité Mixte de Recherche 5004, CNRS/INRA/Montpellier SupAgro/Université Montpellier, F-34060 Montpellier, Cedex 2, France maurel@supagro.inra.fr.
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1532-298X
DOI
10.1105/tpc.15.00421
Email
maurel@supagro.inra.fr
Published
2015-07-00
Epub
2015-00-10
Pages
1945-54
Language
English
Region
England
NLM ID
9208688
PMCID
PMC4531361
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.15.00421 PMC Full Text View journal details
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