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PMID: 2587261 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Structure and functional expression of a cloned Xenopus thyroid hormone receptor.

Nucleic acids research ·Vol. 17 ·No. 22 ·1989-11-25 ·Pages 9395-405

Brooks AR, Sweeney G, Old RW

Abstract

A clone corresponding to a thyroid hormone receptor was isolated from a Xenopus laevis cDNA library prepared from folliculated oocytes. The cDNA encodes a protein of 418 amino acid residues with a domain structure, including a putative DNA binding region with two zinc fingers, similar to other members of the v-erbA-related superfamily of receptors. The encoded protein resembles the TR alpha 1-type receptor of the rat. When expressed in COS cells the protein product binds triiodothyronine with a Kd of 0.12 nM. The receptor mediates thyroid-hormone-inducible expression of a reporter gene which includes a thyroid hormone response element in its upstream region.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Line Cloning, Molecular Gene Expression Gene Library Genes Kinetics Molecular Sequence Data Oocytes/metabolism Protein Biosynthesis Protein Conformation Receptors, Thyroid Hormone/genetics,metabolism Recombinant Proteins/metabolism Restriction Mapping Transcription, Genetic Transfection Xenopus laevis
Chemicals
Receptors, Thyroid Hormone Recombinant Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Brooks A R
Department of Biological Sciences, University of Warwick, Coventry, UK.
Sweeney G
Old R W
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/17.22.9395
Published
1989-11-25
Pages
9395-405
Language
English
Region
England
NLM ID
0411011
PMCID
PMC335140
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/17.22.9395 PMC Full Text View journal details
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