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PMID: 2580304 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Influenza viral (A/WSN/33) hemagglutinin is expressed and glycosylated in the yeast Saccharomyces cerevisiae.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 7 ·1985-04-00 ·Pages 2019-23

Jabbar MA, Sivasubramanian N, Nayak DP

Abstract

Recombinant plasmids were constructed in which genes coding for either the entire or the signal-minus (amino acid residues 2-17 deleted) hemagglutinin (HA) of WSN influenza virus were placed under the control of the alcohol dehydrogenase I gene promoter of Saccharomyces cerevisiae. Both recombinant plasmids were shown to direct the synthesis of HA-specific polypeptides that were detected by immunoprecipitation with antiviral antibodies. The complete HA produced in yeast had an approximate Mr of 70,000 and was glycosylated, as determined by the endoglycosidase H sensitivity, and was bound to membrane. Therefore, the complete HA polypeptide possessing the signal sequence probably traversed the yeast secretory pathways. Signal-minus HA, on the other hand, had a lower molecular weight and was nonglycosylated. The specific binding of yeast HA with antiviral antibodies could be competitively inhibited by influenza viral HA, demonstrating that the HA produced in yeast contained antigenic determinants of the native viral HA.

MeSH Terms

DNA, Recombinant Epitopes/analysis Hemagglutinin Glycoproteins, Influenza Virus Hemagglutinins, Viral/analysis,genetics,metabolism Plasmids Protein Conformation Saccharomyces cerevisiae/genetics,metabolism Transcription, Genetic

Chemicals

DNA, Recombinant Epitopes Hemagglutinin Glycoproteins, Influenza Virus Hemagglutinins, Viral

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Jabbar M A

Sivasubramanian N

Nayak D P

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25 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.82.7.2019

Published

1985-04-00

Pages

2019-23

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC397479

Subset

Databases

GENBANK

M11106

External Links

PubMed source DOI: 10.1073/pnas.82.7.2019 PMC Full Text View journal details

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Influenza viral (A/WSN/33) hemagglutinin is expressed and glycosylated in the yeast Saccharomyces cerevisiae.

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