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PMID: 2577506 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Deletion analysis of the polyadenylation signal of a pea ribulose-1,5-bisphosphate carboxylase small-subunit gene.

Plant molecular biology ·Vol. 13 ·No. 2 ·1989-08-00 ·Pages 125-38

Hunt AG, MacDonald MH

Abstract

The polyadenylation signal of a pea gene for the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcS) has been analyzed by deletion mutagenesis and Ti plasmid-mediated gene transfer. Sequences between 6 and 137 bases upstream from the normal polyadenylation sites in this gene (bases -6 to -137) are required for functioning of these sites. In addition, bases -111 to -235 can affect 3' end formation by altering the pattern of 3' termini seen in various transcription units. Sequences between 37 and 95 bases upstream from a cryptic polyadenylation site in this gene [A. G. Hunt, DNA 7: 329-336 (1988)] are necessary for mRNA 3' end formation at this site. At least two different parts of the 3' region of this rbcS gene can serve as a downstream element for polyadenylation at the normal poly(A) addition sites in this gene. Our studies indicate that: 1. the upstream sequences required for polyadenylation in plants are different from those defined in mammalian RNA polymerase II transcription units; 2. sequences 100 or more bases upstream and downstream from poly(A) addition sites in this gene can affect poly(A) addition site choice; and 3. there are apparently redundant downstream elements for polyadenylation in this gene.

Related Genes
rbcS
MeSH Terms
Base Sequence Chromosome Deletion DNA/genetics DNA Mutational Analysis DNA Probes Fabaceae/genetics,metabolism Molecular Sequence Data Plants/genetics,metabolism Plants, Genetically Modified/genetics,metabolism Plants, Medicinal Plasmids Poly A/genetics,metabolism RNA, Messenger/genetics,metabolism Ribulose-Bisphosphate Carboxylase/genetics,metabolism Transcription, Genetic
Chemicals
DNA Probes RNA, Messenger Poly A DNA Ribulose-Bisphosphate Carboxylase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hunt A G
Department of Agronomy, University of Kentucky, Lexington 40546-0091.
MacDonald M H
References (21)
21 references, click to expand
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00016132
Published
1989-08-00
Pages
125-38
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00016132 View journal details
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