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PMID: 2557588 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cytosine methylation in CTF and Sp1 recognition sites of an HSV tk promoter: effects on transcription in vivo and on factor binding in vitro.

Nucleic acids research ·Vol. 17 ·No. 24 ·1989-12-25 ·Pages 10179-90

Ben-Hattar J, Beard P, Jiricny J

Abstract

We methylated specific cytosine residues within or immediately around the CTF and Sp1 binding sites of the Herpes simplex virus thymidine kinase promoter. The efficiency of transcription in vivo was reduced at least 50-fold compared with transcription from the unmethylated promoter. However, methylation within the CTF recognition site had no effect on the affinity of CTF for this site in vitro. Methylation of the Sp1 site resulted in only a small decrease in the affinity of this factor for its recognition site. In vivo studies showed that the same gene inserted in different vector DNAs was regulated differently by methylation in the promoter. These results show that cytosine methylation can inhibit transcription by a mechanism other than directly blocking the binding of transcription factors.

MeSH Terms
Animals Base Sequence Binding Sites Binding, Competitive Cytosine/metabolism DNA, Viral/genetics,metabolism DNA-Binding Proteins/metabolism HeLa Cells Humans Methylation Molecular Sequence Data Oocytes Promoter Regions, Genetic Simplexvirus/enzymology,genetics Sp1 Transcription Factor Thymidine Kinase/genetics Transcription Factors/metabolism Transcription, Genetic Xenopus laevis
Chemicals
DNA, Viral DNA-Binding Proteins Sp1 Transcription Factor Transcription Factors Cytosine Thymidine Kinase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ben-Hattar J
Swiss Institute for Experimental Cancer Research, Lausanne.
Beard P
Jiricny J
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/17.24.10179
Published
1989-12-25
Pages
10179-90
Language
English
Region
England
NLM ID
0411011
PMCID
PMC335292
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/17.24.10179 PMC Full Text View journal details
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