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PMID: 3707542 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The effect of HhaI methylation on DNA local structure.

The Biochemical journal ·Vol. 234 ·No. 1 ·1986-02-15 ·Pages 213-6

Fox KR

Abstract

The effect of methylating the central cytosine in the sequence GCGC on the sensitivity to digestion by deoxyribonuclease I was investigated. Examination of five such sites shows that the extra methyl group renders the bond on its 5' side more susceptible to cleavage, and this is interpreted by suggesting that it causes small changes in the local DNA phosphate orientation.

MeSH Terms

Base Sequence Binding Sites DNA DNA (Cytosine-5-)-Methyltransferases DNA-Cytosine Methylases Densitometry Deoxyribonuclease I Electrophoresis, Polyacrylamide Gel Methylation Nucleic Acid Conformation

Chemicals

DNA DNA modification methylase HhaI DNA-Cytosine Methylases DNA (Cytosine-5-)-Methyltransferases Deoxyribonuclease I

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Fox K R

References (18)

18 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2340213

Published

1986-02-15

Pages

213-6

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1146547

Subset

External Links

PubMed source DOI: 10.1042/bj2340213 PMC Full Text View journal details

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The effect of HhaI methylation on DNA local structure.

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