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PMID: 2554316 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Translocation of proteins across the mitochondrial inner membrane, but not into the outer membrane, requires nucleoside triphosphates in the matrix.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 21 ·1989-11-00 ·Pages 8432-6

Hwang ST, Schatz G

Abstract

Work in several laboratories has established that import of proteins across the mitochondrial inner membrane requires an electrochemical potential across that membrane and cleavage of nucleoside triphosphate. We now show that nucleoside triphosphate must be present inside the inner membrane. In contrast, the potential-independent insertion of porin into the outer membrane requires ATP only outside the inner membrane.

MeSH Terms
Adenosine Triphosphate/metabolism Bacterial Outer Membrane Proteins/metabolism Guanosine Triphosphate/metabolism Intracellular Membranes/drug effects,metabolism L-Lactate Dehydrogenase/metabolism L-Lactate Dehydrogenase (Cytochrome) Mitochondria/metabolism Porins Potassium Cyanide/pharmacology Proteins/metabolism Recombinant Fusion Proteins/metabolism Ribonucleotides/metabolism Saccharomyces cerevisiae/metabolism Tetrahydrofolate Dehydrogenase/genetics,metabolism Valinomycin/pharmacology
Chemicals
Bacterial Outer Membrane Proteins Porins Proteins Recombinant Fusion Proteins Ribonucleotides Valinomycin Guanosine Triphosphate Adenosine Triphosphate L-Lactate Dehydrogenase L-Lactate Dehydrogenase (Cytochrome) Tetrahydrofolate Dehydrogenase Potassium Cyanide
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hwang S T
Biocenter, University of Basel, Switzerland.
Schatz G
References (28)
28 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.21.8432
Published
1989-11-00
Pages
8432-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC298296
Subset
IM
Grants
NIGMS NIH HHS · CBY-11 ROI GM 37803 · United States
External Links
PubMed source DOI: 10.1073/pnas.86.21.8432 PMC Full Text View journal details
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