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PMID: 2953027 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Protein import into chloroplasts requires a chloroplast ATPase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 10 ·1987-05-00 ·Pages 3288-92

Pain D, Blobel G

Abstract

We have transcribed mRNA from a cDNA clone coding for pea ribulose-1,5-bisphosphate carboxylase, translated the mRNA in a wheat germ cell-free system, and studied the energy requirement for posttranslational import of the [35S]methionine-labeled protein into the stroma of pea chloroplasts. We found that import depends on ATP hydrolysis within the stroma. Import is not inhibited when H+, K+, Na+, or divalent cation gradients across the chloroplast membranes are dissipated by ionophores, as long as exogenously added ATP is also present during the import reaction. Our data suggest that protein import into the chloroplast stroma requires a chloroplast ATPase that does not function to generate a membrane potential for driving the import reaction but that exerts its effect in another, yet-to-be-determined, mode. We have carried out a preliminary characterization of this ATPase regarding its nucleotide specificity and the effects of various ATPase inhibitors.

MeSH Terms
Adenosine Triphosphatases/metabolism Chloroplasts/enzymology DNA/metabolism Kinetics Plants/enzymology,genetics Protein Biosynthesis Protein Processing, Post-Translational RNA, Messenger/genetics Ribulose-Bisphosphate Carboxylase/genetics Transcription, Genetic
Chemicals
RNA, Messenger DNA Adenosine Triphosphatases Ribulose-Bisphosphate Carboxylase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pain D
Blobel G
References (24)
24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.10.3288
Published
1987-05-00
Pages
3288-92
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC304854
Subset
IM
Grants
NIGMS NIH HHS · GM 27155 · United States
External Links
PubMed source DOI: 10.1073/pnas.84.10.3288 PMC Full Text View journal details
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