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PMID: 2841112 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Unfolding and refolding of a purified precursor protein during import into isolated mitochondria.

The EMBO journal ·Vol. 7 ·No. 4 ·1988-04-00 ·Pages 1139-45

Eilers M, Hwang S, Schatz G

Abstract

A purified mitochondrial precursor protein unfolds to a protease-sensitive conformation at the surface of isolated mitochondria before being imported into the organelles. This unfolding is stimulated by a potential across the mitochondrial inner membrane, but does not require ATP. In contrast, import of the surface-bound unfolded precursor requires ATP, but no potential; it is accompanied by a refolding inside the mitochondria.

MeSH Terms

Animals Electron Transport Complex IV/genetics,metabolism Enzyme Precursors/genetics Macromolecular Substances Mice Mitochondria/enzymology Protein Conformation Protein Denaturation Recombinant Fusion Proteins/metabolism Recombinant Proteins/metabolism Saccharomyces cerevisiae/enzymology,genetics Tetrahydrofolate Dehydrogenase/genetics,metabolism Urea/pharmacology

Chemicals

Enzyme Precursors Macromolecular Substances Recombinant Fusion Proteins Recombinant Proteins Urea Tetrahydrofolate Dehydrogenase Electron Transport Complex IV

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Eilers M

University of Basel, Department of Biochemistry, Switzerland.

Hwang S

Schatz G

References (24)

24 references, click to expand

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Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1988-04-00

Pages

1139-45

Language

English

Region

England

NLM ID

8208664

PMCID

PMC454448

Subset

Grants

NIGMS NIH HHS · CBY-1 1 R01 GM37803-01 · United States

External Links

PubMed source PMC Full Text View journal details

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Unfolding and refolding of a purified precursor protein during import into isolated mitochondria.

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