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PMID: 3297670 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The ATP requiring step in assembly of M13 procoat protein into microsomes is related to preservation of transport competence of the precursor protein.

The EMBO journal ·Vol. 6 ·No. 4 ·1987-04-00 ·Pages 1011-6

Wiech H, Sagstetter M, Müller G, Zimmermann R

Abstract

M13 procoat protein is processed to transmembrane coat protein by dog pancreas microsomes after completion of synthesis and in the absence of the signal recognition particle (SRP)/docking protein system. ATP is required for fast and efficient processing of procoat protein by microsomes in a reticulocyte lysate. Requirement for ATP is also observed in the absence of ribosomes or docking protein. This indicates the existence of a unique assembly pathway for procoat protein into microsomes which depends on ATP but does not depend on the SRP/docking protein and ribosome/ribosome receptor systems. We suggest that the ATP requirement is linked to a so far unknown component of the reticulocyte lysate, acting on transport competence of precursor proteins.

MeSH Terms

Adenosine Triphosphate/metabolism Animals Capsid/biosynthesis,genetics Capsid Proteins Coliphages/genetics,metabolism Dogs Endoplasmic Reticulum/metabolism Escherichia coli/genetics,metabolism Microsomes/metabolism Pancreas/metabolism Plasmids Protein Biosynthesis Protein Precursors/biosynthesis,genetics Protein Processing, Post-Translational Rabbits Reticulocytes/metabolism Transcription, Genetic

Chemicals

Capsid Proteins Protein Precursors coat protein, Bacteriophage M13 Adenosine Triphosphate

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Wiech H

Sagstetter M

Müller G

Zimmermann R

References (29)

29 references, click to expand

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Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1987-04-00

Pages

1011-6

Language

English

Region

England

NLM ID

8208664

PMCID

PMC553497

Subset

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PubMed source PMC Full Text View journal details

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The ATP requiring step in assembly of M13 procoat protein into microsomes is related to preservation of transport competence of the precursor protein.

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