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PMID: 6344069 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

M13 procoat and a pre-immunoglobulin share processing specificity but use different membrane receptor mechanisms.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 10 ·1983-05-00 ·Pages 2809-13

Watts C, Wickner W, Zimmermann R

Abstract

Bacteriophage M13 procoat is accurately processed to transmembrane coat protein by salt-washed or N-ethylmaleimide-treated rough microsomes from dog pancreas. These treatments inhibit the processing of eukaryotic secreted protein precursors. M13 procoat can assemble into dog pancreas microsomes post-translationally. Thus, the microsomal proteins needed for assembly may be determined by the nature of the precursor protein itself. These results, and our finding that the mouse IgG kappa chain fragment precursor is processed by Escherichia coli leader peptidase, also suggest that the cleavage specificity of leader (signal) peptidases and the properties of preproteins that render them suitable for cleavage have been conserved during evolution.

MeSH Terms
Animals Biological Evolution Coliphages/genetics Dogs Endopeptidases/metabolism Immunoglobulins/genetics Membrane Proteins Microsomes/metabolism Pancreas/metabolism Protein Precursors/metabolism Protein Processing, Post-Translational Serine Endopeptidases Substrate Specificity Viral Proteins/genetics
Chemicals
Immunoglobulins Membrane Proteins Protein Precursors Viral Proteins Endopeptidases Serine Endopeptidases type I signal peptidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Watts C
Wickner W
Zimmermann R
References (36)
36 references, click to expand
  1. Efficient cleavage and segregation of nascent presecretory proteins in a reticulocyte lysate supplemented with microsomal membranes.
    J Biol Chem. 1978 Jun 10;253(11):3753-6 PMID: 649601
  2. Protein transfer across microsomal membranes reassembled from separated membrane components.
    Nature. 1978 Jun 15;273(5663):569-71 PMID: 96350
  3. Transcription in bacteriophage F1-infected Escherichia coli. I. Translation of the RNA in vitro.
    Virology. 1978 May 15;86(2):368-75 PMID: 351926
  4. Assembly of the Semliki Forest virus membrane glycoproteins in the membrane of the endoplasmic reticulum in vitro.
    J Mol Biol. 1978 Oct 5;124(4):587-600 PMID: 712848
  5. Membrane biogenesis: cotranslational integration of the bacteriophage f1 coat protein into an Escherichia coli membrane fraction.
    Proc Natl Acad Sci U S A. 1979 Mar;76(3):1251-5 PMID: 375232
  6. Synthesis, assembly into the cytoplasmic membrane, and proteolytic processing of the precursor of coliphage M13 coat protein.
    J Biol Chem. 1980 Mar 10;255(5):2123-30 PMID: 6986388
  7. Purification and characterization of leader (signal) peptidase from Escherichia coli.
    J Biol Chem. 1980 Aug 25;255(16):7973-7 PMID: 6995457
  8. Secretion requires a cytoplasmically disposed sulphydryl of the RER membrane.
    Nature. 1980 Jul 10;286(5769):174-6 PMID: 6995850
  9. RNA splicing generates a variant light chain from an aberrantly rearranged kappa gene.
    Nature. 1980 Aug 21;286(5775):776-9 PMID: 6772972
  10. Secretion of beta-lactamase requires the carboxy end of the protein.
    Cell. 1980 Jul;20(3):749-60 PMID: 6448092
  11. A membrane component essential for vectorial translocation of nascent proteins across the endoplasmic reticulum: requirements for its extraction and reassociation with the membrane.
    J Cell Biol. 1980 NOV;87(2 Pt 1):498-502 PMID: 7000796
  12. Procoat, the precursor of M13 coat protein, requires an electrochemical potential for membrane insertion.
    Proc Natl Acad Sci U S A. 1980 Aug;77(8):4669-73 PMID: 7001463
  13. Precursor proteins are intermediates in vivo in the synthesis of two major outer membrane proteins, the OmpA and OmpF proteins, of Escherichia coli K12.
    Eur J Biochem. 1981 Jan;113(2):375-80 PMID: 7009158
  14. Purification of a membrane-associated protein complex required for protein translocation across the endoplasmic reticulum.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7112-6 PMID: 6938958
  15. Procoat, the precursor of M13 coat protein, inserts post-translationally into the membrane of cells infected by wild-type virus.
    J Virol. 1981 Mar;37(3):1087-9 PMID: 7014926
  16. Membrane assembly: posttranslational insertion of M13 procoat protein into E. coli membranes and its proteolytic conversion to coat protein in vitro.
    Cell. 1981 May;24(2):437-41 PMID: 7237555
  17. Energy is required for maturation of exported proteins in Escherichia coli.
    Eur J Biochem. 1981 May 15;116(2):227-33 PMID: 7018904
  18. Different transport pathways of individual precursor proteins in mitochondria.
    Eur J Biochem. 1981 Jun 1;116(3):455-60 PMID: 6167439
  19. Different exported proteins in E. coli show differences in the temporal mode of processing in vivo.
    Cell. 1981 Jul;25(1):151-7 PMID: 7023693
  20. Membrane assembly from purified components. I. Isolated M13 procoat does not require ribosomes or soluble proteins for processing by membranes.
    Cell. 1981 Aug;25(2):341-5 PMID: 7026042
  21. Membrane assembly from purified components. II. Assembly of M13 procoat into liposomes reconstituted with purified leader peptidase.
    Cell. 1981 Aug;25(2):347-53 PMID: 7026043
  22. An 'internal' signal sequence directs secretion and processing or proinsulin in bacteria.
    Nature. 1981 Nov 12;294(5837):176-8 PMID: 7029306
  23. Role for membrane potential in the secretion of protein into the periplasm of Escherichia coli.
    Proc Natl Acad Sci U S A. 1981 Sep;78(9):5396-400 PMID: 7029533
  24. Translocation of proteins across the endoplasmic reticulum III. Signal recognition protein (SRP) causes signal sequence-dependent and site-specific arrest of chain elongation that is released by microsomal membranes.
    J Cell Biol. 1981 Nov;91(2 Pt 1):557-61 PMID: 7309797
  25. Characterization of molecules involved in protein translocation using a specific antibody.
    J Cell Biol. 1982 Feb;92(2):579-83 PMID: 7037796
  26. The isolation of homogeneous leader peptidase from a strain of Escherichia coli which overproduces the enzyme.
    J Biol Chem. 1982 Jul 10;257(13):7898-902 PMID: 6282859
  27. Secretory protein translocation across membranes-the role of the "docking protein'.
    Nature. 1982 Jun 24;297(5868):647-50 PMID: 7088152
  28. [Virus proteins. IV. Constitution of the coat protein of the fd phage].
    Hoppe Seylers Z Physiol Chem. 1969 Sep;350(9):1047-66 PMID: 5349375
  29. Synthesis, assembly, and secretion of gamma globulin by mouse myeloma cells. I. Adaptation of the Merwin plasma cell tumor-11 to culture, cloning, and characterization of gamma globulin subunits.
    J Exp Med. 1970 Mar 1;131(3):515-41 PMID: 4189836
  30. Transfer of proteins across membranes. II. Reconstitution of functional rough microsomes from heterologous components.
    J Cell Biol. 1975 Dec;67(3):852-62 PMID: 811672
  31. Asymmetric orientation of phage M13 coat protein in Escherichia coli cytoplasmic membranes and in synthetic lipid vesicles.
    Proc Natl Acad Sci U S A. 1976 Apr;73(4):1159-63 PMID: 772680
  32. Studies on bacteriophage fd DNA. IV. The sequence of messenger RNA for the major coat protein gene.
    J Mol Biol. 1977 Apr 25;111(4):487-507 PMID: 864706
  33. Cloned MPC 11 myeloma cells express two kappa genes: a gene for a complete light chain and a gene for a constant region polypeptide.
    Cell. 1977 Oct;12(2):453-62 PMID: 410502
  34. Synchronised transmembrane insertion and glycosylation of a nascent membrane protein.
    Nature. 1977 Oct 27;269(5631):775-80 PMID: 200844
  35. Transfer of proteins across membranes, Biosynthesis in vitro of pretrypsinogen and trypsinogen by cell fractions of canine pancreas.
    Eur J Biochem. 1978 Jan 16;82(2):593-9 PMID: 624289
  36. Synthesis of phage M13 coat protein and its assembly into membranes in vitro.
    Proc Natl Acad Sci U S A. 1978 Apr;75(4):1754-8 PMID: 273906
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.10.2809
Published
1983-05-00
Pages
2809-13
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC393921
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.80.10.2809 PMC Full Text View journal details
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