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PMID: 2549000 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Genetic and molecular analyses of the gene encoding staphylococcal enterotoxin D.

Journal of bacteriology ·Vol. 171 ·No. 9 ·1989-09-00 ·Pages 4799-806

Bayles KW, Iandolo JJ

Abstract

The gene (entD) encoding staphylococcal enterotoxin D (SED) has been located on a 27.6-kilobase penicillinase plasmid designated pIB485. This plasmid was present in all SED-producing strains tested. The entD gene was cloned on a 2.0-kilobase DNA fragment and was expressed in Escherichia coli. Sequence analysis of this fragment revealed an open reading frame that encoded a 258-amino-acid protein that possessed a 30-amino-acid signal peptide. The 228-amino-acid mature polypeptide had a molecular weight of 26,360 and contained a high degree of sequence similarity to the other staphylococcal enterotoxins. S1 nuclease mapping showed that transcription of entD was initiated 266 nucleotides upstream from the translation start codon. The entD gene was also shown to be activated by the staphylococcal regulatory element known as agr.

MeSH Terms
Amino Acid Sequence Base Sequence Endonucleases Enterotoxins/genetics Escherichia coli/genetics Genes Genes, Bacterial Genotype Molecular Sequence Data Plasmids Repetitive Sequences, Nucleic Acid Restriction Mapping Sequence Homology, Nucleic Acid Single-Strand Specific DNA and RNA Endonucleases Staphylococcus aureus/genetics Transcription, Genetic
Chemicals
Enterotoxins enterotoxin D, Staphylococcal Endonucleases Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bayles K W
Division of Biology, Kansas State University, Manhattan 66506.
Iandolo J J
References (42)
42 references, click to expand
  1. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  2. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  3. Purification and some physicochemical properties of staphylococcal enterotoxin D.
    Biochemistry. 1979 May 15;18(10):1937-42 PMID: 107962
  4. Genetics of staphylococcal enterotoxin B in methicillin-resistant isolates of Staphylococcus aureus.
    Infect Immun. 1979 Sep;25(3):902-11 PMID: 259057
  5. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  6. Regulatory sequences involved in the promotion and termination of RNA transcription.
    Annu Rev Genet. 1979;13:319-53 PMID: 94251
  7. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  8. Identification of a chromosomal determinant of alpha-toxin production in Staphylococcus aureus.
    Infect Immun. 1980 Oct;30(1):36-42 PMID: 6254884
  9. Plasmid-chromosomal transition of genes important in staphylococcal enterotoxin B expression.
    Infect Immun. 1981 Aug;33(2):450-8 PMID: 6792077
  10. A rapid boiling method for the preparation of bacterial plasmids.
    Anal Biochem. 1981 Jun;114(1):193-7 PMID: 6269464
  11. Chromosomal mapping of a gene affecting enterotoxin A production in Staphylococcus aureus.
    Appl Environ Microbiol. 1982 Feb;43(2):397-402 PMID: 6277247
  12. Rapid similarity searches of nucleic acid and protein data banks.
    Proc Natl Acad Sci U S A. 1983 Feb;80(3):726-30 PMID: 6572363
  13. Compilation and analysis of Escherichia coli promoter DNA sequences.
    Nucleic Acids Res. 1983 Apr 25;11(8):2237-55 PMID: 6344016
  14. A genetic system for analysis of staphylococcal nuclease.
    Gene. 1983 May-Jun;22(2-3):181-9 PMID: 6307819
  15. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  16. Staphylococcal enterotoxin A gene is associated with a variable genetic element.
    Proc Natl Acad Sci U S A. 1984 Aug;81(16):5179-83 PMID: 6089183
  17. Penicillinase plasmid-linked genetic determinants for enterotoxins B and C1 production in Staphylococcus aureus.
    Infect Immun. 1985 Feb;47(2):514-21 PMID: 3871425
  18. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  19. A rapid single-stranded cloning strategy for producing a sequential series of overlapping clones for use in DNA sequencing: application to sequencing the corn mitochondrial 18 S rDNA.
    Plasmid. 1985 Jan;13(1):31-40 PMID: 3991809
  20. Staphylococcal enterotoxin A is encoded by phage.
    Science. 1985 Jul 12;229(4709):185-7 PMID: 3160112
  21. Nucleotide sequence of the type A streptococcal exotoxin (erythrogenic toxin) gene from Streptococcus pyogenes bacteriophage T12.
    Infect Immun. 1986 Apr;52(1):144-50 PMID: 3514452
  22. Nucleotide sequence of the enterotoxin B gene from Staphylococcus aureus.
    J Bacteriol. 1986 Apr;166(1):29-33 PMID: 3957869
  23. Regulation of exoprotein gene expression in Staphylococcus aureus by agar.
    Mol Gen Genet. 1986 Jan;202(1):58-61 PMID: 3007938
  24. Lysogenic conversion of staphylococcal lipase is caused by insertion of the bacteriophage L54a genome into the lipase structural gene.
    J Bacteriol. 1986 May;166(2):385-91 PMID: 3009394
  25. Integration of staphylococcal phage L54a occurs by site-specific recombination: structural analysis of the attachment sites.
    Proc Natl Acad Sci U S A. 1986 Aug;83(15):5474-8 PMID: 2942938
  26. Sequence determination and comparison of the exfoliative toxin A and toxin B genes from Staphylococcus aureus.
    J Bacteriol. 1987 Sep;169(9):3904-9 PMID: 3040666
  27. Nucleotide sequence of the epidermolytic toxin A gene of Staphylococcus aureus.
    J Bacteriol. 1987 Sep;169(9):3910-5 PMID: 3040667
  28. Nucleotide sequence of the staphylococcal enterotoxin C1 gene and relatedness to other pyrogenic toxins.
    Mol Gen Genet. 1987 Aug;209(1):15-20 PMID: 2823067
  29. Nucleotide sequence of the type A staphylococcal enterotoxin gene.
    J Bacteriol. 1988 Jan;170(1):34-41 PMID: 3335483
  30. Regulation of the enterotoxin B gene in Staphylococcus aureus.
    J Biol Chem. 1988 May 5;263(13):6276-80 PMID: 3360783
  31. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  32. Isolation of extrachromosomal deoxyribonucleic acid for exfoliative toxin production from phage group II Staphylococcus aureus.
    J Bacteriol. 1975 Apr;122(1):99-105 PMID: 123526
  33. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
    Cell. 1977 Nov;12(3):721-32 PMID: 922889
  34. Cloning of a chromosomal locus (exp) which regulates the expression of several exoprotein genes in Staphylococcus aureus.
    Mol Gen Genet. 1988 Mar;211(3):435-40 PMID: 3285138
  35. Cloning and nucleotide sequence of the type E staphylococcal enterotoxin gene.
    J Bacteriol. 1988 Jul;170(7):2954-60 PMID: 3384800
  36. Staphylococcal enterotoxin B gene is associated with a discrete genetic element.
    J Bacteriol. 1988 Sep;170(9):4033-9 PMID: 2842299
  37. Cloning, characterization, and sequencing of an accessory gene regulator (agr) in Staphylococcus aureus.
    J Bacteriol. 1988 Sep;170(9):4365-72 PMID: 2457579
  38. Posttranscriptional regulatory mechanisms in Escherichia coli.
    Annu Rev Biochem. 1988;57:199-233 PMID: 3052271
  39. Retroregulation of the bacteriophage lambda int gene: limited secondary degradation of the RNase III-processed transcript.
    J Bacteriol. 1989 Jan;171(1):588-92 PMID: 2521618
  40. Inactivation of the alpha-haemolysin gene of Staphylococcus aureus 8325-4 by site-directed mutagenesis and studies on the expression of its haemolysins.
    Microb Pathog. 1986 Apr;1(2):125-38 PMID: 3508485
  41. Molecular cloning and expression of the epidermolytic toxin A gene of Staphylococcus aureus.
    Microb Pathog. 1986 Dec;1(6):583-94 PMID: 3508500
  42. High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA.
    Mol Gen Genet. 1979 Jan 5;168(1):111-5 PMID: 107388
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.9.4799-4806.1989
Published
1989-09-00
Pages
4799-806
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC210282
Subset
IM
Grants
NIAID NIH HHS · AI-17474 · United States
Databases
GENBANK
M28521
External Links
PubMed source DOI: 10.1128/jb.171.9.4799-4806.1989 PMC Full Text View journal details
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