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PMID: 2548852 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

GABAA receptor beta subunit heterogeneity: functional expression of cloned cDNAs.

The EMBO journal ·Vol. 8 ·No. 6 ·1989-06-00 ·Pages 1665-70

Ymer S, Schofield PR, Draguhn A, Werner P, Köhler M, Seeburg PH

Abstract

Cloned cDNAs encoding two new beta subunits of the rat and bovine GABAA receptor have been isolated using a degenerate oligonucleotide probe based on a highly conserved peptide sequence in the second transmembrane domain of GABAA receptor subunits. The beta 2 and beta 3 subunits share approximately 72% sequence identity with the previously characterized beta 1 polypeptide. Northern analysis showed that both beta 2 and beta 3 mRNAs are more abundant in the brain than beta 1 mRNA. All three beta subunit encoding cDNAs were also identified in a library constructed from adrenal medulla RNA. Each beta subunit, when co-expressed in Xenopus oocytes with an alpha subunit, forms functional GABAA receptors. These results, together with the known alpha subunit heterogeneity, suggest that a variety of related but functionally distinct GABAA receptor subtypes are generated by different subunit combinations.

MeSH Terms
Adrenal Medulla/metabolism Amino Acid Sequence Animals Base Sequence Brain/metabolism Cattle Cloning, Molecular DNA/genetics Gene Expression Regulation Molecular Sequence Data Oocytes/metabolism Protein Conformation Rats Receptors, GABA-A/genetics,metabolism Sequence Homology, Nucleic Acid Xenopus
Chemicals
Receptors, GABA-A DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Ymer S
Laboratory of Molecular Neuroendocrinology, University of Heidelberg, FRG.
Schofield P R
Draguhn A
Werner P
Köhler M
Seeburg P H
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1989-06-00
Pages
1665-70
Language
English
Region
England
NLM ID
8208664
PMCID
PMC401007
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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