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PMID: 2546931 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Review

The muscle ryanodine receptor and its intrinsic Ca2+ channel activity.

Journal of bioenergetics and biomembranes ·Vol. 21 ·No. 2 ·1989-04-00 ·Pages 227-46

Lai FA, Meissner G

Abstract

In skeletal and cardiac muscle, contraction is initiated by the rapid release of Ca2+ ions from the intracellular membrane system, sarcoplasmic reticulum. Rapid-mixing vesicle ion flux and planar lipid bilayer-single-channel measurements have shown that Ca2+ release is mediated by a high-conductance, ligand-gated Ca2+ channel. Using the Ca2+ release-specific probe ryanodine, a 30 S protein complex composed of four polypeptides of Mr approximately 400,000 has been isolated. Reconstitution of the purified skeletal and cardiac muscle 30 S complexes into planar lipid bilayers induced single Ca2+ channel currents with conductance and gating kinetics similar to those of native Ca2+ release channels. Electron microscopy revealed structural similarity with the protein bridges ("feet") that span the transverse-tubule-sarcoplasmic reticulum junction. These results suggest that striated muscle contains an intracellular Ca2+ release channel that is identical with the ryanodine receptor and the transverse-tubule-sarcoplasmic reticulum spanning feet structures.

MeSH Terms
Animals Calcium/physiology Calcium Channels/physiology Muscles/physiology Receptors, Cholinergic/isolation & purification,physiology Ryanodine Receptor Calcium Release Channel
Chemicals
Calcium Channels Receptors, Cholinergic Ryanodine Receptor Calcium Release Channel Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lai F A
Department of Biochemistry, School of Medicine, University of North Carolina, Chapel Hill 27599-7260.
Meissner G
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Article Info
Journal
Journal of bioenergetics and biomembranes
Abbr.
J Bioenerg Biomembr
ISSN
0145-479X
DOI
10.1007/BF00812070
Published
1989-04-00
Pages
227-46
Language
English
Region
United States
NLM ID
7701859
Subset
IM
Grants
NIAMS NIH HHS · AR18687 · United States
NHLBI NIH HHS · HL27430 · United States
NHLBI NIH HHS · HL38835 · United States
External Links
PubMed source DOI: 10.1007/BF00812070 View journal details
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