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PMID: 2543631 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of porin and ompR mutants of a virulent strain of Salmonella typhimurium: ompR mutants are attenuated in vivo.

Infection and immunity ·Vol. 57 ·No. 7 ·1989-07-00 ·Pages 2136-40

Dorman CJ, Chatfield S, Higgins CF, Hayward C, Dougan G

Abstract

The ompC, ompD, and ompF genes encode the three major porins of Salmonella typhimurium. ompR encodes a positive regulator required for the expression of ompC and ompF. Transposon-generated mutations in ompC, ompD, ompF, and ompR were introduced into the S. typhimurium mouse virulent strain SL1344 by P22-mediated transduction. Following preliminary characterization in vitro, the strains were used to challenge BALB/c mice by using the oral or intravenous route. Strains harboring ompC or ompF mutations were as virulent as SL1344 after oral challenge. Strains harboring ompD mutations had a slight reduction in virulence. In contrast, ompR mutants failed to kill BALB/c mice after oral challenge and the intravenous 50% lethal dose was reduced by approximately 10(5). The ompR mutants persisted in murine tissues for several weeks following oral or intravenous challenge. Furthermore, mice orally immunized with these ompR mutant strains were well protected against challenge with virulent SL1344.

MeSH Terms
Administration, Oral Animals Bacterial Outer Membrane Proteins/genetics Bacterial Proteins/genetics Injections, Intravenous Male Mice Mice, Inbred BALB C Mutation Osmolar Concentration Porins Salmonella typhimurium/genetics,growth & development,pathogenicity Vaccines, Attenuated/administration & dosage,genetics Virulence
Chemicals
Bacterial Outer Membrane Proteins Bacterial Proteins Porins Vaccines, Attenuated
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Dorman C J
Department of Biochemistry, University of Dundee, United Kingdom.
Chatfield S
Higgins C F
Hayward C
Dougan G
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43 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.57.7.2136-2140.1989
Published
1989-07-00
Pages
2136-40
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC313852
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.57.7.2136-2140.1989 PMC Full Text View journal details
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