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PMID: 2542892 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Dual function of a nuclear factor I binding site in MMTV transcription regulation.

Nucleic acids research ·Vol. 17 ·No. 8 ·1989-04-25 ·Pages 3065-78

Buetti E, Kühnel B, Diggelmann H

Abstract

Using linker-scanning mutagenesis we had previously identified four elements within the MMTV LTR which are necessary for transcriptional stimulation by glucocorticoid hormones. Two of them overlapped with regions to which the glucocorticoid receptor binds in vitro. The third element contained a NF-I binding site, and the fourth the TATA box. Here we show that mutations that abolish in vitro binding of NF-I had a negative effect also on the basal activity of the MMTV promoter of LTR-containing plasmids stably integrated in Ltk- fibroblasts. The analysis of double mutants altered in the NF-I plus either one of the receptor binding elements further demonstrated that the NF-I site functionally cooperated with the proximal (-120) element, which alone was extremely inefficient in stimulation. The stronger distal (-181/-172) element was independent of NF-I and showed functional cooperativity with the proximal hormone-binding element.

MeSH Terms
Animals Binding Sites CCAAT-Enhancer-Binding Proteins DNA Mutational Analysis DNA-Binding Proteins/physiology Dexamethasone/pharmacology Gene Expression Regulation/drug effects L Cells Mammary Tumor Virus, Mouse/genetics Mice NFI Transcription Factors Nuclear Proteins/physiology Promoter Regions, Genetic Receptors, Glucocorticoid/physiology Regulatory Sequences, Nucleic Acid Transcription Factors/physiology Transcription, Genetic Y-Box-Binding Protein 1
Chemicals
CCAAT-Enhancer-Binding Proteins DNA-Binding Proteins NFI Transcription Factors Nuclear Proteins Receptors, Glucocorticoid Transcription Factors Y-Box-Binding Protein 1 YBX1 protein, human Dexamethasone
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Buetti E
Swiss Institute for Experimental Cancer Research, Epalinges.
Kühnel B
Diggelmann H
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/17.8.3065
Published
1989-04-25
Pages
3065-78
Language
English
Region
England
NLM ID
0411011
PMCID
PMC317714
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/17.8.3065 PMC Full Text View journal details
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