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PMID: 2542766 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Control of Saccharomyces cerevisiae catalase T gene (CTT1) expression by nutrient supply via the RAS-cyclic AMP pathway.

Molecular and cellular biology ·Vol. 9 ·No. 3 ·1989-03-00 ·Pages 1309-15

Bissinger PH, Wieser R, Hamilton B, Ruis H

Abstract

In Saccharomyces cerevisiae, lack of nutrients triggers a pleiotropic response characterized by accumulation of storage carbohydrates, early G1 arrest, and sporulation of a/alpha diploids. This response is thought to be mediated by RAS proteins, adenylate cyclase, and cyclic AMP (cAMP)-dependent protein kinases. This study shows that expression of the S. cerevisiae gene coding for a cytoplasmic catalase T (CTT1) is controlled by this pathway: it is regulated by the availability of nutrients. Lack of a nitrogen, sulfur, or phosphorus source causes a high-level expression of the gene. Studies with strains with mutations in the RAS-cAMP pathway and supplementation of a rca1 mutant with cAMP show that CTT1 expression is under negative control by a cAMP-dependent protein kinase and that nutrient control of CTT1 gene expression is mediated by this pathway. Strains containing a CTT1-Escherichia coli lacZ fusion gene have been used to isolate mutants with mutations in the pathway. Mutants characterized in this investigation fall into five complementation groups. Both cdc25 and ras2 alleles were identified among these mutants.

MeSH Terms
Adenylyl Cyclases/metabolism Catalase/genetics Cyclic AMP/metabolism Fungal Proteins/metabolism Gene Expression Regulation Genes, Fungal Mutation Protein Kinases/metabolism Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins ras Proteins
Chemicals
Fungal Proteins Saccharomyces cerevisiae Proteins Cyclic AMP Catalase Protein Kinases RAS2 protein, S cerevisiae ras Proteins Adenylyl Cyclases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bissinger P H
Institut für Allgemeine Biochemie, Universität Wien, Austria.
Wieser R
Hamilton B
Ruis H
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.3.1309-1315.1989
Published
1989-03-00
Pages
1309-15
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362723
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.9.3.1309-1315.1989 PMC Full Text View journal details
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