Home LiteratureArticle Details
PMID: 2835229 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Dual regulation of the expression of the polyubiquitin gene by cyclic AMP and heat shock in yeast.

The EMBO journal ·Vol. 7 ·No. 2 ·1988-02-00 ·Pages 495-502

Tanaka K, Matsumoto K, Toh-e A

Abstract

The expression of the polyubiquitin gene in yeast, UB14, is repressed by cAMP-dependent protein phosphorylation. Since cycloheximide does not inhibit the induction of the UB14 transcription, no de novo protein synthesis is required in this process. The expression of the UB14 gene is also induced by mild heat shock by a mechanism other than depletion of cAMP. From the genetic analysis of the UB14 gene, we propose that the UB14 gene is one of the genes which are part of the cAMP-effector pathway and required for G0/G1 arrest in Saccharomyces cerevisiae.

MeSH Terms
Cell Cycle Cyclic AMP/pharmacology Gene Expression Regulation Genes/drug effects Genes, Fungal/drug effects Genotype Hot Temperature Kinetics Mutation Phenotype Saccharomyces cerevisiae/cytology,drug effects,genetics Transcription, Genetic/drug effects Ubiquitins/genetics
Chemicals
Ubiquitins Cyclic AMP
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tanaka K
Department of Fermentation Technology, Hiroshima University, Japan.
Matsumoto K
Toh-e A
References (36)
36 references, click to expand
  1. Isolation of a polypeptide that has lymphocyte-differentiating properties and is probably represented universally in living cells.
    Proc Natl Acad Sci U S A. 1975 Jan;72(1):11-5 PMID: 1078892
  2. Isolation and characterization of a phosphoprotein phosphatase-deficient mutant in yeast.
    Yeast. 1985 Sep;1(1):25-38 PMID: 2851899
  3. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  4. The primary structure of a glyceraldehyde-3-phosphate dehydrogenase gene from Saccharomyces cerevisiae.
    J Biol Chem. 1979 Oct 10;254(19):9839-45 PMID: 385592
  5. Ubiquitin is the ATP-dependent proteolysis factor I of rabbit reticulocytes.
    J Biol Chem. 1980 Aug 25;255(16):7529-32 PMID: 6249803
  6. Molecular cloning of the actin gene from yeast Saccharomyces cerevisiae.
    Nucleic Acids Res. 1980 Mar 11;8(5):1043-59 PMID: 7003553
  7. Isolation and characterization of yeast mutants deficient in adenylate cyclase and cAMP-dependent protein kinase.
    Proc Natl Acad Sci U S A. 1982 Apr;79(7):2355-9 PMID: 6285379
  8. Formation of conjugates by 125I-labelled ubiquitin microinjected into cultured hepatoma cells.
    FEBS Lett. 1982 Jun 1;142(1):72-6 PMID: 6286347
  9. Isolation, characterization, and amino acid sequence of a ubiquitin-like protein from insect eggs.
    J Biol Chem. 1982 Sep 10;257(17):10267-70 PMID: 6286647
  10. Mechanisms of intracellular protein breakdown.
    Annu Rev Biochem. 1982;51:335-64 PMID: 6287917
  11. Transformation of intact yeast cells treated with alkali cations.
    J Bacteriol. 1983 Jan;153(1):163-8 PMID: 6336730
  12. Initiation of meiosis in yeast mutants defective in adenylate cyclase and cyclic AMP-dependent protein kinase.
    Cell. 1983 Feb;32(2):417-23 PMID: 6297794
  13. The nucleotide sequence of the yeast PHO5 gene: a putative precursor of repressible acid phosphatase contains a signal peptide.
    Nucleic Acids Res. 1983 Mar 25;11(6):1657-72 PMID: 6300772
  14. Regulation of yeast mating-type interconversion: feedback control of HO gene expression by the mating-type locus.
    Proc Natl Acad Sci U S A. 1983 May;80(10):3035-9 PMID: 6344075
  15. Studies on transformation of Escherichia coli with plasmids.
    J Mol Biol. 1983 Jun 5;166(4):557-80 PMID: 6345791
  16. One-step gene disruption in yeast.
    Methods Enzymol. 1983;101:202-11 PMID: 6310324
  17. Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors.
    Methods Enzymol. 1983;100:468-500 PMID: 6225933
  18. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  19. Specific early-G1 blocks accompanied with stringent response in Saccharomyces cerevisiae lead to growth arrest in resting state similar to the G0 of higher eucaryotes.
    J Cell Biol. 1984 Apr;98(4):1185-93 PMID: 6371018
  20. Durable synthesis of high molecular weight heat shock proteins in G0 cells of the yeast and other eucaryotes.
    J Cell Biol. 1984 Jul;99(1 Pt 1):199-207 PMID: 6429154
  21. The yeast ubiquitin gene: head-to-tail repeats encoding a polyubiquitin precursor protein.
    Nature. 1984 Dec 13-19;312(5995):663-6 PMID: 6095120
  22. In yeast, RAS proteins are controlling elements of adenylate cyclase.
    Cell. 1985 Jan;40(1):27-36 PMID: 2981630
  23. Ubiquitin is a heat shock protein in chicken embryo fibroblasts.
    Mol Cell Biol. 1985 May;5(5):949-56 PMID: 2987683
  24. The preferential translation of Drosophila hsp70 mRNA requires sequences in the untranslated leader.
    Cell. 1985 Oct;42(3):903-11 PMID: 4053186
  25. DNA sequence and characterization of the S. cerevisiae gene encoding adenylate cyclase.
    Cell. 1985 Dec;43(2 Pt 1):493-505 PMID: 2934138
  26. Translational and transcriptional control elements in the untranslated leader of the heat-shock gene hsp22.
    Cell. 1986 Feb 14;44(3):429-38 PMID: 3943132
  27. A yeast gene that is essential for release from glucose repression encodes a protein kinase.
    Science. 1986 Sep 12;233(4769):1175-80 PMID: 3526554
  28. Identification of a cyclic-AMP-responsive element within the rat somatostatin gene.
    Proc Natl Acad Sci U S A. 1986 Sep;83(18):6682-6 PMID: 2875459
  29. A cyclic AMP- and phorbol ester-inducible DNA element.
    Nature. 1986 Sep 25-Oct 1;323(6086):353-6 PMID: 3020428
  30. Structure and activities of a variant ubiquitin sequence from bakers' yeast.
    Biochemistry. 1986 Sep 9;25(18):4999-5004 PMID: 3021209
  31. The yeast polyubiquitin gene is essential for resistance to high temperatures, starvation, and other stresses.
    Cell. 1987 Mar 27;48(6):1035-46 PMID: 3030556
  32. Heat shock response of Saccharomyces cerevisiae mutants altered in cyclic AMP-dependent protein phosphorylation.
    Mol Cell Biol. 1987 Jan;7(1):244-50 PMID: 3031463
  33. Mutant regulatory subunit of 3',5'-cAMP-dependent protein kinase of yeast Saccharomyces cerevisiae.
    Mol Gen Genet. 1987 Dec;210(3):413-8 PMID: 2828890
  34. Cloning and sequencing of the PHO80 gene and CEN15 of Saccharomyces cerevisiae.
    Yeast. 1986 Jun;2(2):129-39 PMID: 3333302
  35. Genetic analysis of the role of cAMP in yeast.
    Yeast. 1985 Sep;1(1):15-24 PMID: 2851898
  36. Interaction of super-repressible and dominant constitutive mutations for the synthesis of galactose pathway enzymes in Saccharomyces cerevisiae.
    Mol Gen Genet. 1977 Apr 29;152(3):137-44 PMID: 327270
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1988-02-00
Pages
495-502
Language
English
Region
England
NLM ID
8208664
PMCID
PMC454346
Subset
IM
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2