Home LiteratureArticle Details
PMID: 2541436 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

denV gene of bacteriophage T4 restores DNA excision repair to mei-9 and mus201 mutants of Drosophila melanogaster.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 9 ·1989-05-00 ·Pages 3227-31

Banga SS, Boyd JB, Valerie K, Harris PV, Kurz EM, de Riel JK

Abstract

The denV gene of bacteriophage T4 was fused to a Drosophila hsp70 (70-kDa heat shock protein) promoter and introduced into the germ line of Drosophila by P-element-mediated transformation. The protein product of that gene (endonuclease V) was detected in extracts of heat-shocked transformants with both enzymological and immunoblotting procedures. That protein restores both excision repair and UV resistance to mei-9 and mus201 mutants of this organism. These results reveal that the denV gene can compensate for excision-repair defects in two very different eukaryotic mutants, in that the mus201 mutants are typical of excision-deficient mutants in other organisms, whereas the mei-9 mutants exhibit a broad pleiotropism that includes a strong meiotic deficiency. This study permits an extension of the molecular analysis of DNA repair to the germ line of higher eukaryotes. It also provides a model system for future investigations of other well-characterized microbial repair genes on DNA damage in the germ line of this metazoan organism.

MeSH Terms
Animals Blotting, Western Cloning, Molecular DNA/biosynthesis DNA Glycosylases DNA Repair DNA Restriction Enzymes DNA, Recombinant Deoxyribonuclease (Pyrimidine Dimer) Drosophila melanogaster/genetics Endodeoxyribonucleases/genetics Gene Expression Regulation Genes, Viral Germ Cells Heat-Shock Proteins/genetics Mutation N-Glycosyl Hydrolases/metabolism Plasmids Promoter Regions, Genetic T-Phages/genetics Transformation, Genetic
Chemicals
DNA, Recombinant Heat-Shock Proteins DNA Endodeoxyribonucleases DNA Restriction Enzymes Deoxyribonuclease (Pyrimidine Dimer) DNA Glycosylases N-Glycosyl Hydrolases deoxyribopyrimidine endonucleosidase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Banga S S
Department of Genetics, University of California, Davis 95616.
Boyd J B
Valerie K
Harris P V
Kurz E M
de Riel J K
References (27)
27 references, click to expand
  1. Excision repair in Drosophila. Analysis of strand breaks appearing in DNA of mei-9 mutants following mutagen treatment.
    Biochim Biophys Acta. 1980 Nov 14;610(1):116-29 PMID: 6776988
  2. The molecular biology of nucleotide excision repair of DNA: recent progress.
    J Cell Sci Suppl. 1987;6:1-23 PMID: 2821018
  3. Effects of radiation on the survival of excision-defective cells from Drosophila melanogaster.
    Somatic Cell Genet. 1981 Nov;7(6):631-44 PMID: 6798701
  4. Expression of the denV gene of coliphage T4 in UV-sensitive rad mutants of Saccharomyces cerevisiae.
    Mol Cell Biol. 1986 Oct;6(10):3559-62 PMID: 3540595
  5. Physical association of pyrimidine dimer DNA glycosylase and apurinic/apyrimidinic DNA endonuclease essential for repair of ultraviolet-damaged DNA.
    Proc Natl Acad Sci U S A. 1981 May;78(5):2742-6 PMID: 6265906
  6. Photoreactivation rescue and hypermutability of ultraviolet-irradiated excisionless Drosophila melanogaster larvae.
    Proc Natl Acad Sci U S A. 1986 May;83(10):3366-70 PMID: 3085088
  7. New heat shock puffs and beta-galactosidase activity resulting from transformation of Drosophila with an hsp70-lacZ hybrid gene.
    Cell. 1983 Dec;35(2 Pt 1):403-10 PMID: 6418386
  8. Expression of the bacteriophage T4 denV structural gene in Escherichia coli.
    J Bacteriol. 1986 Nov;168(2):1014-8 PMID: 3536845
  9. Expression of a cloned denV gene of bacteriophage T4 in Escherichia coli.
    Proc Natl Acad Sci U S A. 1985 Jul;82(14):4763-7 PMID: 2991891
  10. Identification of a second locus in Drosophila melanogaster required for excision repair.
    Genetics. 1982 Feb;100(2):239-57 PMID: 6809529
  11. Isolation and characterization of a photorepair-deficient mutant in Drosophila melanogaster.
    Genetics. 1987 Jun;116(2):233-9 PMID: 3111935
  12. Transient and stable complementation of ultraviolet repair in xeroderma pigmentosum cells by the denV gene of bacteriophage T4.
    Cancer Res. 1987 Jun 1;47(11):2967-71 PMID: 3567913
  13. Genetic transformation of Drosophila with transposable element vectors.
    Science. 1982 Oct 22;218(4570):348-53 PMID: 6289436
  14. A transposable P vector that confers selectable G418 resistance to Drosophila larvae.
    EMBO J. 1985 Jan;4(1):167-71 PMID: 16453599
  15. Correct developmental expression of a cloned alcohol dehydrogenase gene transduced into the Drosophila germ line.
    Cell. 1983 Aug;34(1):59-73 PMID: 6309412
  16. den V gene of bacteriophage T4 determines a DNA glycosylase specific for pyrimidine dimers in DNA.
    J Virol. 1980 Sep;35(3):790-6 PMID: 6252338
  17. Genetic complementation of UV-induced DNA repair in Chinese hamster ovary cells by the denV gene of phage T4.
    Proc Natl Acad Sci U S A. 1985 Nov;82(22):7656-60 PMID: 3865186
  18. Production and detection of coliphage T4 endonuclease V polyclonal and monoclonal antibodies using staphylococcal protein-A hybrid proteins.
    Gene. 1987;58(1):99-107 PMID: 2446955
  19. The heat-shock response.
    Annu Rev Biochem. 1986;55:1151-91 PMID: 2427013
  20. Identification, physical map location and sequence of the denV gene from bacteriophage T4.
    Nucleic Acids Res. 1984 Nov 12;12(21):8085-96 PMID: 6095188
  21. A genetic and molecular analysis of DNA repair in Drosophila.
    J Cell Sci Suppl. 1987;6:39-60 PMID: 3308924
  22. The proportionality between mutation rate and ultraviolet dose after photoreactivation in Drosophila.
    Genetics. 1962 Mar;47:361-6 PMID: 13873780
  23. Physical mapping and complete nucleotide sequence of the denV gene of bacteriophage T4.
    J Virol. 1984 Dec;52(3):846-56 PMID: 6092716
  24. Partial complementation of the UV sensitivity of E. coli and yeast excision repair mutants by the cloned denV gene of bacteriophage T4.
    Mol Gen Genet. 1986 Apr;203(1):163-71 PMID: 3520242
  25. Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis.
    Gene. 1983 Dec;26(1):101-6 PMID: 6323249
  26. Restoration of u.v.-induced excision repair in Xeroderma D cells transfected with the denV gene of bacteriophage T4.
    EMBO J. 1987 Oct;6(10):3125-31 PMID: 3319581
  27. Expression of the mouse dihydrofolate reductase complementary deoxyribonucleic acid in simian virus 40 vectors.
    Mol Cell Biol. 1981 Sep;1(9):854-64 PMID: 9279398
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.9.3227
Published
1989-05-00
Pages
3227-31
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC287103
Subset
IM
Grants
NIGMS NIH HHS · GM32040 · United States
External Links
PubMed source DOI: 10.1073/pnas.86.9.3227 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2