Home LiteratureArticle Details
PMID: 3536845 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Expression of the bacteriophage T4 denV structural gene in Escherichia coli.

Journal of bacteriology ·Vol. 168 ·No. 2 ·1986-11-00 ·Pages 1014-8

Recinos A, Augustine ML, Higgins KM, Lloyd RS

Abstract

The expression of the T4 denV gene, which previously had been cloned in plasmid constructs downstream of the bacteriophage lambda hybrid promoter-operator oLpR, was analyzed under a variety of growth parameters. Expression of the denV gene product, endonuclease V, was confirmed in DNA repair-deficient Escherichia coli (uvrA recA) by Western blot analyses and by enhancements of resistance to UV irradiation.

MeSH Terms
Cloning, Molecular DNA, Recombinant Deoxyribonuclease (Pyrimidine Dimer) Endodeoxyribonucleases/biosynthesis,genetics Escherichia coli/enzymology,genetics,radiation effects Genes Genes, Viral Plasmids T-Phages/enzymology,genetics Ultraviolet Rays
Chemicals
DNA, Recombinant Endodeoxyribonucleases Deoxyribonuclease (Pyrimidine Dimer)
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Recinos A
Augustine M L
Higgins K M
Lloyd R S
References (20)
20 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. Efficient screening of recombinant DNA junctions afforded by probing with synthetic "bridge" oligonucleotides.
    Biochem Biophys Res Commun. 1986 Jul 31;138(2):945-52 PMID: 3017346
  3. DNA repair of ultraviolet-irradiated bacteriophage T4.
    Photochem Photobiol. 1975 Apr;21(4):277-89 PMID: 1108058
  4. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  5. A pyrimidine dimer-DNA glycosylase activity associated with the v gene product of bacterophage T4.
    Nature. 1980 Jul 10;286(5769):182-5 PMID: 6250044
  6. den V gene of bacteriophage T4 determines a DNA glycosylase specific for pyrimidine dimers in DNA.
    J Virol. 1980 Sep;35(3):790-6 PMID: 6252338
  7. Comparison of the cleavage of pyrimidine dimers by the bacteriophage T4 and Micrococcus luteus UV-specific endonucleases.
    J Biol Chem. 1980 Dec 25;255(24):12047-50 PMID: 6254991
  8. Codon catalog usage is a genome strategy modulated for gene expressivity.
    Nucleic Acids Res. 1981 Jan 10;9(1):r43-74 PMID: 7208352
  9. Physical association of pyrimidine dimer DNA glycosylase and apurinic/apyrimidinic DNA endonuclease essential for repair of ultraviolet-damaged DNA.
    Proc Natl Acad Sci U S A. 1981 May;78(5):2742-6 PMID: 6265906
  10. Expression of the denV gene of bacteriophage T4 cloned in Escherichia coli.
    Proc Natl Acad Sci U S A. 1981 May;78(5):2796-800 PMID: 6265912
  11. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.
    Anal Biochem. 1981 Apr;112(2):195-203 PMID: 6266278
  12. Evidence that the UV endonuclease activity induced by bacteriophage T4 contains both pyrimidine dimer-DNA glycosylase and apyrimidinic/apurinic endonuclease activities in the enzyme molecule.
    J Virol. 1981 Oct;40(1):204-10 PMID: 6270374
  13. den V gene of bacteriophage T4 codes for both pyrimidine dimer-DNA glycosylase and apyrimidinic endonuclease activities.
    J Virol. 1981 Oct;40(1):211-23 PMID: 6270375
  14. Purification and characterization of normal and mutant forms of T4 endonuclease V.
    J Biol Chem. 1982 Mar 10;257(5):2556-62 PMID: 6277906
  15. Physical mapping and complete nucleotide sequence of the denV gene of bacteriophage T4.
    J Virol. 1984 Dec;52(3):846-56 PMID: 6092716
  16. Identification, physical map location and sequence of the denV gene from bacteriophage T4.
    Nucleic Acids Res. 1984 Nov 12;12(21):8085-96 PMID: 6095188
  17. Expression of a cloned denV gene of bacteriophage T4 in Escherichia coli.
    Proc Natl Acad Sci U S A. 1985 Jul;82(14):4763-7 PMID: 2991891
  18. Cloning and expression of the 3' portion of the T4 denV gene as a lacZ fusion gene.
    Mutat Res. 1986 Mar;165(2):89-100 PMID: 3081797
  19. Partial complementation of the UV sensitivity of E. coli and yeast excision repair mutants by the cloned denV gene of bacteriophage T4.
    Mol Gen Genet. 1986 Apr;203(1):163-71 PMID: 3520242
  20. Pedigrees of some mutant strains of Escherichia coli K-12.
    Bacteriol Rev. 1972 Dec;36(4):525-57 PMID: 4568763
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.168.2.1014-1018.1986
Published
1986-11-00
Pages
1014-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC213586
Subset
IM
Grants
NIEHS NIH HHS · ES 00267 · United States
External Links
PubMed source DOI: 10.1128/jb.168.2.1014-1018.1986 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2