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PMID: 2536836 Published · ppublish English Journal Article

Genetic basis of attenuation of the Sabin type 3 oral poliovirus vaccine.

Journal of virology ·Vol. 63 ·No. 3 ·1989-03-00 ·Pages 1338-44

Westrop GD, Wareham KA, Evans DM, Dunn G, Minor PD, Magrath DI, Taffs F, Marsden S, Skinner MA, Schild GC

Abstract

The poliovirus type 3 Sabin oral poliovirus vaccine strain P3/Leon/12a1b differs in nucleotide sequence from its neurovirulent progenitor P3/Leon/37 by just 10 point mutations. The contribution of each mutation to the attenuation phenotype of the vaccine strain was determined by the construction of a series of recombinant viruses from infectious cDNA clones. The neurovirulence testing of recombinant viruses indicated that the attenuation phenotype is determined by just two point mutations: a C to U in the noncoding region at position 472 and a C to U at nucleotide 2034 which results in a serine-to-phenylalanine amino acid substitution in the structural protein VP3.

MeSH Terms
Amino Acid Sequence Animals Biological Assay Cloning, Molecular DNA/genetics DNA Mutational Analysis Genes, Viral Nervous System/microbiology Poliovirus/genetics,pathogenicity Poliovirus Vaccine, Oral/genetics RNA, Viral/genetics Regulatory Sequences, Nucleic Acid Structure-Activity Relationship Vaccines, Attenuated/genetics
Chemicals
Poliovirus Vaccine, Oral RNA, Viral Vaccines, Attenuated DNA
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Westrop G D
Department of Microbiology, University of Reading, United Kingdom.
Wareham K A
Evans D M
Dunn G
Minor P D
Magrath D I
Taffs F
Marsden S
Skinner M A
Schild G C
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.63.3.1338-1344.1989
Published
1989-03-00
Pages
1338-44
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC247831
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.63.3.1338-1344.1989 PMC Full Text View journal details
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