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PMID: 2536828 Published · ppublish English Journal Article

Mutational analysis of the conserved basic domain of human immunodeficiency virus tat protein.

Journal of virology ·Vol. 63 ·No. 3 ·1989-03-00 ·Pages 1181-7

Hauber J, Malim MH, Cullen BR

Abstract

The tat trans-activators encoded by the known strains of primate immunodeficiency virus share a conserved, highly basic protein domain. Mutagenesis of this sequence in the tat gene of human immunodeficiency virus type 1 is shown here to reduce, but not eliminate, the trans-activation of human immunodeficiency virus type 1-specific gene expression. The degree of inhibition is shown to vary in a dose-dependent manner and is most marked at low levels of tat expression. Multiple mutations of the basic domain of tat were found to impair both the in vivo stability and the nuclear localization of the tat protein. It is proposed that this protein domain serves to efficiently target the tat gene product to its appropriate site or substrate within the nucleus of expressing cells.

MeSH Terms
Cell Compartmentation DNA Mutational Analysis Fluorescent Antibody Technique Gene Expression Regulation Gene Products, tat HIV/genetics,metabolism Nuclear Proteins/genetics Precipitin Tests Retroviridae Proteins/genetics,metabolism Structure-Activity Relationship Transcription Factors/genetics tat Gene Products, Human Immunodeficiency Virus
Chemicals
Gene Products, tat Nuclear Proteins Retroviridae Proteins Transcription Factors tat Gene Products, Human Immunodeficiency Virus
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hauber J
Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina 27710.
Malim M H
Cullen B R
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36 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.63.3.1181-1187.1989
Published
1989-03-00
Pages
1181-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC247813
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.63.3.1181-1187.1989 PMC Full Text View journal details
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