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PMID: 25330236 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

Ethylene-induced inhibition of root growth requires abscisic acid function in rice (Oryza sativa L.) seedlings.

PLoS genetics ·Vol. 10 ·No. 10 ·2014-10-00 ·Pages e1004701

Ma B, Yin CC, He SJ, Lu X, Zhang WK, Lu TG, Chen SY, Zhang JS

Abstract

Ethylene and abscisic acid (ABA) have a complicated interplay in many developmental processes. Their interaction in rice is largely unclear. Here, we characterized a rice ethylene-response mutant mhz4, which exhibited reduced ethylene-response in roots but enhanced ethylene-response in coleoptiles of etiolated seedlings. MHZ4 was identified through map-based cloning and encoded a chloroplast-localized membrane protein homologous to Arabidopsis thaliana (Arabidopsis) ABA4, which is responsible for a branch of ABA biosynthesis. MHZ4 mutation reduced ABA level, but promoted ethylene production. Ethylene induced MHZ4 expression and promoted ABA accumulation in roots. MHZ4 overexpression resulted in enhanced and reduced ethylene response in roots and coleoptiles, respectively. In root, MHZ4-dependent ABA pathway acts at or downstream of ethylene receptors and positively regulates root ethylene response. This ethylene-ABA interaction mode is different from that reported in Arabidopsis, where ethylene-mediated root inhibition is independent of ABA function. In coleoptile, MHZ4-dependent ABA pathway acts at or upstream of OsEIN2 to negatively regulate coleoptile ethylene response, possibly by affecting OsEIN2 expression. At mature stage, mhz4 mutation affects branching and adventitious root formation on stem nodes of higher positions, as well as yield-related traits. Together, our findings reveal a novel mode of interplay between ethylene and ABA in control of rice growth and development.

MeSH Terms
Abscisic Acid/metabolism Arabidopsis Proteins/metabolism Cotyledon/drug effects,metabolism Ethylenes/metabolism,pharmacology Gene Expression Regulation, Plant Mutation Oryza/drug effects,genetics,growth & development Phylogeny Plant Proteins/genetics,metabolism Plant Roots/genetics,growth & development,metabolism Plants, Genetically Modified Seedlings/genetics,growth & development,metabolism Sequence Homology, Amino Acid
Chemicals
Arabidopsis Proteins Ethylenes Plant Proteins Abscisic Acid ethylene
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Ma Biao
State Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
Yin Cui-Cui
State Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
He Si-Jie
State Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
Lu Xiang
State Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
Zhang Wan-Ke
State Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
Lu Tie-Gang
Biotechnology Research Institute/National Key Facility for Genetic Resources and Gene Improvement, Chinese Academy of Agricultural Sciences, Beijing, China.
Chen Shou-Yi
State Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
Zhang Jin-Song
State Key Lab of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
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Article Info
Journal
PLoS genetics
Abbr.
PLoS Genet
ISSN
1553-7404
DOI
10.1371/journal.pgen.1004701
Published
2014-10-00
Epub
2014-00-16
Pages
e1004701
Language
English
Region
United States
NLM ID
101239074
PMCID
PMC4199509
Subset
IM
External Links
PubMed source DOI: 10.1371/journal.pgen.1004701 PMC Full Text View journal details
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