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PMID: 22451723 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Modulation of ethylene responses by OsRTH1 overexpression reveals the biological significance of ethylene in rice seedling growth and development.

Journal of experimental botany ·Vol. 63 ·No. 11 ·2012-06-00 ·Pages 4151-64

Zhang W, Zhou X, Wen CK

Abstract

Overexpression of Arabidopsis Reversion-To-ethylene Sensitivity1 (RTE1) results in whole-plant ethylene insensitivity dependent on the ethylene receptor gene Ethylene Response1 (ETR1). However, overexpression of the tomato RTE1 homologue Green Ripe (GR) delays fruit ripening but does not confer whole-plant ethylene insensitivity. It was decided to investigate whether aspects of ethylene-induced growth and development of the monocotyledonous model plant rice could be modulated by rice RTE1 homologues (OsRTH genes). Results from a cross-species complementation test in Arabidopsis showed that OsRTH1 overexpression complemented the rte1-2 loss-of-function mutation and conferred whole-plant ethylene insensitivity in an ETR1-dependent manner. In contrast, OsRTH2 and OsRTH3 overexpression did not complement rte1-2 or confer ethylene insensitivity. In rice, OsRTH1 overexpression substantially prevented ethylene-induced alterations in growth and development, including leaf senescence, seedling leaf elongation and development, coleoptile elongation or curvature, and adventitious root development. Results of subcellular localizations of OsRTHs, each fused with the green fluorescent protein, in onion epidermal cells suggested that the three OsRTHs were predominantly localized to the Golgi. OsRTH1 may be an RTE1 orthologue of rice and modulate rice ethylene responses. The possible roles of auxins and gibberellins in the ethylene-induced alterations in growth were evaluated and the biological significance of ethylene in the early stage of rice seedling growth is discussed.

MeSH Terms
Amino Acid Sequence Arabidopsis/genetics,growth & development,metabolism Ethylenes/metabolism Gene Expression Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Molecular Sequence Data Oryza/chemistry,genetics,growth & development,metabolism Plant Growth Regulators/metabolism Plant Proteins/chemistry,genetics,metabolism Seedlings/genetics,growth & development,metabolism Sequence Alignment
Chemicals
Ethylenes Plant Growth Regulators Plant Proteins ethylene
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Zhang Wei
National Key Laboratory of Plant Molecular Genetics and National Center for Plant Gene Research (Shanghai), Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
Zhou Xin
Wen Chi-Kuang
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Article Info
Journal
Journal of experimental botany
Abbr.
J Exp Bot
ISSN
1460-2431
DOI
10.1093/jxb/ers098
Published
2012-06-00
Epub
2012-00-26
Pages
4151-64
Language
English
Region
England
NLM ID
9882906
PMCID
PMC3398448
Subset
IM
External Links
PubMed source DOI: 10.1093/jxb/ers098 PMC Full Text View journal details
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