Home LiteratureArticle Details

PMID: 2532362 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cloning of the cDNA and gene for a human D2 dopamine receptor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 24 ·1989-12-00 ·Pages 9762-6

Grandy DK, Marchionni MA, Makam H, Stofko RE, Alfano M, Frothingham L, Fischer JB, Burke-Howie KJ, Bunzow JR, Server AC

Abstract

A clone encoding a human D2 dopamine receptor was isolated from a pituitary cDNA library and sequenced. The deduced protein sequence is 96% identical with that of the cloned rat receptor with one major difference: the human receptor contains an additional 29 amino acids in its putative third cytoplasmic loop. Southern blotting demonstrated the presence of only one human D2 receptor gene. Two overlapping phage containing the gene were isolated and characterized. DNA sequence analysis of these clones showed that the coding sequence is interrupted by six introns and that the additional amino acids present in the human pituitary receptor are encoded by a single exon of 87 base pairs. The involvement of this sequence in alternative splicing and its biological significance are discussed.

MeSH Terms

Amino Acid Sequence Animals Base Sequence Binding, Competitive Blotting, Southern Cloning, Molecular Corpus Striatum/metabolism DNA/genetics Domperidone/metabolism Exons Gene Library Genes Humans Kinetics Molecular Sequence Data Pituitary Gland/metabolism Rats Receptors, Dopamine/genetics,metabolism Receptors, Dopamine D2 Restriction Mapping Sequence Homology, Nucleic Acid

Chemicals

Receptors, Dopamine Receptors, Dopamine D2 Domperidone DNA

Authors & Affiliations

10 authors, click to expand affiliations / ORCID

Grandy D K

Vollum Institute for Advanced Biomedical Research, Oregon Health Sciences University, Portland 97201.

Marchionni M A

Makam H

Stofko R E

Alfano M

Frothingham L

Fischer J B

Burke-Howie K J

Bunzow J R

Server A C

References (25)

25 references, click to expand

Dopamine inhibits adenylate cyclase in human prolactin-secreting pituitary adenomas.
Nature. 1979 Mar 15;278(5701):252-4 PMID: 423973
Role of brain monoamines and histamine in regulation of anterior pituitary secretion.
Physiol Rev. 1978 Oct;58(4):905-76 PMID: 212770
High efficiency DNA-mediated transformation of primate cells.
Science. 1983 Aug 5;221(4610):551-3 PMID: 6306768
Isolation, sequence analysis, and intron-exon arrangement of the gene encoding bovine rhodopsin.
Cell. 1983 Oct;34(3):807-14 PMID: 6194890
Genomic sequencing.
Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991-5 PMID: 6326095
Bimodal distribution of dopamine receptor densities in brains of schizophrenics.
Science. 1984 Aug 17;225(4663):728-31 PMID: 6147018
The Drosophila ninaE gene encodes an opsin.
Cell. 1985 Apr;40(4):839-50 PMID: 2985266
[3H]-domperidone labels only a single population of receptors which convert from high to low affinity for dopamine in rat brain.
Naunyn Schmiedebergs Arch Pharmacol. 1986 Jan;332(1):21-5 PMID: 2869417
The triosephosphate isomerase gene from maize: introns antedate the plant-animal divergence.
Cell. 1986 Jul 4;46(1):133-41 PMID: 3755078
Structure and organization of the chicken H2B histone gene family.
Nucleic Acids Res. 1987 Feb 11;15(3):1063-80 PMID: 3822819
Primary structure and biochemical properties of an M2 muscarinic receptor.
Science. 1987 May 1;236(4801):600-5 PMID: 3107123
A family of receptors coupled to guanine nucleotide regulatory proteins.
Biochemistry. 1987 May 19;26(10):2657-64 PMID: 3038163
Mutations that uncouple the beta-adrenergic receptor from Gs and increase agonist affinity.
J Biol Chem. 1987 Dec 5;262(34):16439-43 PMID: 2890637
The bombesin receptor is coupled to a guanine nucleotide-binding protein which is insensitive to pertussis and cholera toxins.
J Biol Chem. 1988 Feb 25;263(6):2808-16 PMID: 2830264
D1 dopamine receptor--the search for a function: a critical evaluation of the D1/D2 dopamine receptor classification and its functional implications.
Synapse. 1987;1(4):347-88 PMID: 2971273
Cloning and expression of a rat D2 dopamine receptor cDNA.
Nature. 1988 Dec 22-29;336(6201):783-7 PMID: 2974511
Structure of the adrenergic and related receptors.
Annu Rev Neurosci. 1989;12:67-83 PMID: 2648960
Palmitoylation of the human beta 2-adrenergic receptor. Mutation of Cys341 in the carboxyl tail leads to an uncoupled nonpalmitoylated form of the receptor.
J Biol Chem. 1989 May 5;264(13):7564-9 PMID: 2540197
Mechanisms of signal transduction at the dopamine D2 receptor.
Trends Pharmacol Sci. 1989 Feb;10(2):74-7 PMID: 2655242
Antipsychotic drugs: direct correlation between clinical potency and presynaptic action on dopamine neurons.
Science. 1975 Jun 20;188(4194):1217-9 PMID: 1145194
Dopamine receptor binding predicts clinical and pharmacological potencies of antischizophrenic drugs.
Science. 1976 Apr 30;192(4238):481-3 PMID: 3854
Why genes in pieces?
Nature. 1978 Feb 9;271(5645):501 PMID: 622185
Receptor basis for dopaminergic supersensitivity in Parkinson's disease.
Nature. 1978 May 4;273(5657):59-61 PMID: 692671
Dopamine-sensitive adenylyl cyclase: a receptor mechanism for dopamine.
Adv Biochem Psychopharmacol. 1978;19:131-54 PMID: 29445
3H-Domperidone: a selective ligand for dopamine receptors.
Naunyn Schmiedebergs Arch Pharmacol. 1979 Sep;308(3):231-7 PMID: 503250

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.86.24.9762

Published

1989-12-00

Pages

9762-6

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC298581

Subset

Grants

NIDDK NIH HHS · DK37231 · United States

NIMH NIH HHS · MH45614 · United States

Databases

GENBANK

M29066

External Links

PubMed source DOI: 10.1073/pnas.86.24.9762 PMC Full Text View journal details

Analysis Services

Cloning of the cDNA and gene for a human D2 dopamine receptor.

Contact

WeChat Official Account