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PMID: 2523514 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The adenovirus-inducible factor E2F stimulates transcription after specific DNA binding.

Molecular and cellular biology ·Vol. 9 ·No. 2 ·1989-02-00 ·Pages 578-85

Yee AS, Raychaudhuri P, Jakoi L, Nevins JR

Abstract

The promoter-specific factor E2F interacts with critical regulatory sequences within the adenovirus E2 promoter. In addition, the level of active factor increases markedly during a virus infection, dependent on E1A function and coincident with the trans activation of E2 transcription. We have purified the E2F factor through a combination of standard biochemical procedures and DNA affinity chromatography. The purified factor was a single polypeptide of 54,000 molecular weight, as determined by UV crosslinking and renaturation of gel-fractionated protein. Addition of affinity-purified factor to an in vitro transcription system resulted in stimulation of transcription from a promoter containing two E2F-binding sites but not promoters lacking binding sites. We thus conclude that E2F is indeed capable of stimulating transcription once it has bound to the promoter.

MeSH Terms
Adenovirus Early Proteins Binding Sites DNA/metabolism DNA-Binding Proteins/isolation & purification,metabolism,pharmacology HeLa Cells/drug effects,metabolism Humans Oncogene Proteins, Viral/isolation & purification,metabolism,pharmacology Promoter Regions, Genetic/drug effects Transcription, Genetic/drug effects
Chemicals
Adenovirus Early Proteins DNA-Binding Proteins Oncogene Proteins, Viral DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Yee A S
Rockefeller University, New York, New York 10021.
Raychaudhuri P
Jakoi L
Nevins J R
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42 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.2.578-585.1989
Published
1989-02-00
Pages
578-85
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362634
Subset
IM
Grants
NIGMS NIH HHS · GM26765 · United States
External Links
PubMed source DOI: 10.1128/mcb.9.2.578-585.1989 PMC Full Text View journal details
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