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PMID: 2515276 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Demonstration of H+- and Na+-coupled co-transport of beta-alanine by luminal membrane vesicles of rabbit proximal tubule.

The Journal of physiology ·Vol. 411 ·1989-04-00 ·Pages 517-28

Jessen H, Jørgensen KE, Røigaard-Petersen H, Sheikh MI

Abstract

1. The characteristics of renal transport of beta-alanine by luminal membrane vesicles isolated from either the proximal convoluted part (pars convoluta) or the proximal straight part (pars recta) of rabbit proximal tubule were investigated. 2. In vesicles from pars convoluta two transport systems have been characterized: (1) a Na+-dependent system with intermediate affinity (half-saturation 2.7 mM), and (2) a Na+-independent system, which in the presence of a H+ gradient (extravesicular greater than intravesicular) can drive the uphill transport of beta-alanine into these vesicles. This is the first demonstration of H+-beta-alanine co-transport across luminal membrane of rabbit kidney proximal convoluted tubule. 3. By contrast, in membrane vesicles from pars recta, transport of beta-alanine was strictly dependent on Na+ and occurred via a dual transport system, namely a high-affinity (half-saturation 0.16 mM) and a low-affinity system (half-saturation 9.3 mM). 4. The demonstration of competition between the Na+-gradient-dependent uptake of beta-alanine and taurine, without appreciable inhibition by alpha-amino acids in vesicles from pars convoluta as well as from pars recta, strongly suggests that the luminal membrane of proximal tubule has transport systems for the reabsorption of beta-amino acids which are distinct from alpha-amino acid transport systems.

MeSH Terms
Alanine/pharmacokinetics Amino Acids/pharmacology Animals Biological Transport, Active/drug effects Cations/pharmacology Female Hydrogen-Ion Concentration In Vitro Techniques Kidney Tubules, Proximal/metabolism Kinetics Male Rabbits Sodium/pharmacokinetics beta-Alanine/pharmacokinetics
Chemicals
Amino Acids Cations beta-Alanine Sodium Alanine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Jessen H
Institute of Medical Biochemistry, University of Aarhus, Denmark.
Jørgensen K E
Røigaard-Petersen H
Sheikh M I
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Article Info
Journal
The Journal of physiology
Abbr.
J Physiol
ISSN
0022-3751
DOI
10.1113/jphysiol.1989.sp017587
Published
1989-04-00
Pages
517-28
Language
English
Region
England
NLM ID
0266262
PMCID
PMC1190538
Subset
IM
External Links
PubMed source DOI: 10.1113/jphysiol.1989.sp017587 PMC Full Text View journal details
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