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PMID: 2513484 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

A gamma interferon-unresponsive variant of cell line 70Z/3, IFN-4, can be partially rescued by phorbol myristate acetate.

Molecular and cellular biology ·Vol. 9 ·No. 11 ·1989-11-00 ·Pages 5231-3

Emery DW, Rooney JW, Sibley CH

Abstract

Lipopolysaccharide (LPS) and gamma interferon (IFN-gamma) induce kappa transcription in 70Z/3 cells by different mechanisms; LPS treatment induces both NF-kappa B and OTF-2 transcription factors, but IFN-gamma treatment does not. We have dissected these two activation pathways by selecting and analyzing an LPS+ IFN- variant of 70Z/3.

MeSH Terms
Animals Blotting, Northern Cell Line DNA-Binding Proteins Drug Resistance Genes, Immunoglobulin Immunoglobulin kappa-Chains/genetics Interferon-gamma/pharmacology Lipopolysaccharides/pharmacology NF-kappa B Tetradecanoylphorbol Acetate/pharmacology Transcription Factors/genetics
Chemicals
DNA-Binding Proteins Immunoglobulin kappa-Chains Lipopolysaccharides NF-kappa B Transcription Factors Interferon-gamma Tetradecanoylphorbol Acetate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Emery D W
Department of Genetics, University of Washington, Seattle 98195.
Rooney J W
Sibley C H
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23 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.11.5231-5233.1989
Published
1989-11-00
Pages
5231-3
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363678
Subset
IM
Grants
NCI NIH HHS · CA 29679 · United States
NIGMS NIH HHS · GM 07735 · United States
External Links
PubMed source DOI: 10.1128/mcb.9.11.5231-5233.1989 PMC Full Text View journal details
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