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PMID: 25037212 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

The apical actin fringe contributes to localized cell wall deposition and polarized growth in the lily pollen tube.

Plant physiology ·Vol. 166 ·No. 1 ·2014-09-00 ·Pages 139-51

Rounds CM, Hepler PK, Winship LJ

Abstract

In lily (Lilium formosanum) pollen tubes, pectin, a major component of the cell wall, is delivered through regulated exocytosis. The targeted transport and secretion of the pectin-containing vesicles may be controlled by the cortical actin fringe at the pollen tube apex. Here, we address the role of the actin fringe using three different inhibitors of growth: brefeldin A, latrunculin B, and potassium cyanide. Brefeldin A blocks membrane trafficking and inhibits exocytosis in pollen tubes; it also leads to the degradation of the actin fringe and the formation of an aggregate of filamentous actin at the base of the clear zone. Latrunculin B, which depolymerizes filamentous actin, markedly slows growth but allows focused pectin deposition to continue. Of note, the locus of deposition shifts frequently and correlates with changes in the direction of growth. Finally, potassium cyanide, an electron transport chain inhibitor, briefly stops growth while causing the actin fringe to completely disappear. Pectin deposition continues but lacks focus, instead being delivered in a wide arc across the pollen tube tip. These data support a model in which the actin fringe contributes to the focused secretion of pectin to the apical cell wall and, thus, to the polarized growth of the pollen tube.

MeSH Terms
Actins/metabolism Body Patterning Brefeldin A Bridged Bicyclo Compounds, Heterocyclic Cell Wall/metabolism Lilium/growth & development,metabolism Pectins/metabolism Pollen Tube/growth & development Potassium Cyanide Thiazolidines
Chemicals
Actins Bridged Bicyclo Compounds, Heterocyclic Thiazolidines Brefeldin A Pectins latrunculin B Potassium Cyanide
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rounds Caleb M
Department of Biology, University of Massachusetts, Amherst, Massachusetts 01003 (C.M.R., P.K.H.); andSchool of Natural Science, Hampshire College, Amherst, Massachusetts 01002 (L.J.W.).
Hepler Peter K
Department of Biology, University of Massachusetts, Amherst, Massachusetts 01003 (C.M.R., P.K.H.); andSchool of Natural Science, Hampshire College, Amherst, Massachusetts 01002 (L.J.W.) hepler@bio.umass.edu.
Winship Lawrence J
Department of Biology, University of Massachusetts, Amherst, Massachusetts 01003 (C.M.R., P.K.H.); andSchool of Natural Science, Hampshire College, Amherst, Massachusetts 01002 (L.J.W.).
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
1532-2548
DOI
10.1104/pp.114.242974
Email
hepler@bio.umass.edu
Published
2014-09-00
Epub
2014-00-18
Pages
139-51
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC4149702
Subset
IM
External Links
PubMed source DOI: 10.1104/pp.114.242974 PMC Full Text View journal details
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