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PMID: 19861555 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Exocytosis precedes and predicts the increase in growth in oscillating pollen tubes.

The Plant cell ·Vol. 21 ·No. 10 ·2009-10-00 ·Pages 3026-40

McKenna ST, Kunkel JG, Bosch M, Rounds CM, Vidali L, Winship LJ, Hepler PK

Abstract

We examined exocytosis during oscillatory growth in lily (Lilium formosanum and Lilium longiflorum) and tobacco (Nicotiana tabacum) pollen tubes using three markers: (1) changes in cell wall thickness by Nomarski differential interference contrast (DIC), (2) changes in apical cell wall fluorescence in cells stained with propidium iodide (PI), and (3) changes in apical wall fluorescence in cells expressing tobacco pectin methyl esterase fused to green fluorescent protein (PME-GFP). Using PI fluorescence, we quantified oscillatory changes in the amount of wall material from both lily and tobacco pollen tubes. Measurement of wall thickness by DIC was only possible with lily due to limitations of microscope resolution. PME-GFP, a direct marker for exocytosis, only provides information in tobacco because its expression in lily causes growth inhibition and cell death. We show that exocytosis in pollen tubes oscillates and leads the increase in growth rate; the mean phase difference between exocytosis and growth is -98 degrees +/- 3 degrees in lily and -124 degrees +/- 4 degrees in tobacco. Statistical analyses reveal that the anticipatory increase in wall material predicts, to a high degree, the rate and extent of the subsequent growth surge. Exocytosis emerges as a prime candidate for the initiation and regulation of oscillatory pollen tube growth.

MeSH Terms
Carboxylic Ester Hydrolases/genetics,metabolism Cell Wall/metabolism Exocytosis/genetics,physiology Green Fluorescent Proteins/genetics,metabolism Lilium/genetics,growth & development,metabolism Molecular Sequence Data Pollen Tube/genetics,growth & development,metabolism Tobacco/genetics,growth & development,metabolism
Chemicals
Green Fluorescent Proteins Carboxylic Ester Hydrolases pectinesterase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
McKenna Sylvester T
Department of Biology, Long Island University, Brooklyn, New York 11201, USA.
Kunkel Joseph G
Bosch Maurice
Rounds Caleb M
Vidali Luis
Winship Lawrence J
Hepler Peter K
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1532-298X
DOI
10.1105/tpc.109.069260
Published
2009-10-00
Epub
2009-00-27
Pages
3026-40
Language
English
Region
England
NLM ID
9208688
PMCID
PMC2782290
Subset
IM
Databases
GENBANK
AY772945
External Links
PubMed source DOI: 10.1105/tpc.109.069260 PMC Full Text View journal details
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