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PMID: 2499870 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Pseudorevertants of a lac promoter mutation reveal overlapping nascent promoters.

Nucleic acids research ·Vol. 17 ·No. 10 ·1989-05-25 ·Pages 3927-49

Karls R, Schulz V, Jovanovich SB, Flynn S, Pak A, Reznikoff WS

Abstract

Four pseudorevertants of a -10 region lacP mutation were isolated. Three of these mutations were found to activate nascent promoters. These mutations were: a -2 G/C----A/T change (-2A) promoting transcription at position +11, a +1 A/T----T/A change (+1T) promoting transcription initiation at position +13, and a +10 C/G----A/T change (+10A) promoting transcription initiation at a complex series of positions. The fourth mutation [a -12 T/A----A/T change (-12A)] promotes transcription initiation at -1. The promoters activated by mutations -12A, -2A and +1T resembled the canonical sigma 70 promoter sequences. The +10A promoter activity is also dependent upon the sigma 70 holoenzyme but can not be readily assigned to a specific promoter sequence.

MeSH Terms
Escherichia coli/genetics Genes Genes, Bacterial Genotype Mutation Plasmids Promoter Regions, Genetic Transcription, Genetic beta-Galactosidase/genetics
Chemicals
beta-Galactosidase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Karls R
Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison 53706.
Schulz V
Jovanovich S B
Flynn S
Pak A
Reznikoff W S
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/17.10.3927
Published
1989-05-25
Pages
3927-49
Language
English
Region
England
NLM ID
0411011
PMCID
PMC317870
Subset
IM
Grants
NIGMS NIH HHS · GM 19670 · United States
External Links
PubMed source DOI: 10.1093/nar/17.10.3927 PMC Full Text View journal details
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