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PMID: 2477486 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The human mannose-binding protein gene. Exon structure reveals its evolutionary relationship to a human pulmonary surfactant gene and localization to chromosome 10.

The Journal of experimental medicine ·Vol. 170 ·No. 4 ·1989-10-01 ·Pages 1175-89

Sastry K, Herman GA, Day L, Deignan E, Bruns G, Morton CC, Ezekowitz RA

Abstract

The human mannose-binding protein (MBP) plays a role in first line host defense against certain pathogens. It is an acute phase protein that exists in serum as a multimer of a 32-kD subunit. The NH2 terminus is rich in cysteines that mediate interchain disulphide bonds and stabilize the second collagen-like region. This is followed by a short intervening region, and the carbohydrate recognition domain is found in the COOH-terminal region. Analysis of the human MBP gene reveals that the coding region is interrupted by three introns, and all four exons appear to encode a distinct domain of the protein. It appears that the human MBP gene has evolved by recombination of an ancestral nonfibrillar collagen gene with a gene that encodes carbohydrate recognition, and is therefore similar to the human surfactant SP-A gene and the rat MBP gene. The gene for MBP is located on the long arm of chromosome 10 at 10q11.2-q21, a region that is included in the assignment for the gene for multiple endocrine neoplasia type 2A.

MeSH Terms
Acute-Phase Proteins/genetics,ultrastructure Amino Acid Sequence Base Sequence Biological Evolution Blotting, Southern Carrier Proteins/genetics Chromosome Mapping Chromosomes, Human, Pair 10 Exons Humans Mannose-Binding Lectins Molecular Sequence Data Pulmonary Surfactants/genetics Restriction Mapping Structure-Activity Relationship
Chemicals
Acute-Phase Proteins Carrier Proteins Mannose-Binding Lectins Pulmonary Surfactants
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Sastry K
Division of Hematology/Oncology, Children's Hospital, Boston, Massachusetts 02115.
Herman G A
Day L
Deignan E
Bruns G
Morton C C
Ezekowitz R A
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.170.4.1175
Published
1989-10-01
Pages
1175-89
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2189467
Subset
IM
Grants
NIAID NIH HHS · R01 AI-23786 · United States
Databases
GENBANK
X15422
External Links
PubMed source DOI: 10.1084/jem.170.4.1175 PMC Full Text View journal details
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