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PMID: 2469767 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The human mannose-binding protein functions as an opsonin.

The Journal of experimental medicine ·Vol. 169 ·No. 5 ·1989-05-01 ·Pages 1733-45

Kuhlman M, Joiner K, Ezekowitz RA

Abstract

The human mannose-binding protein (MBP) is a multimeric serum protein that is divided into three domains: a cysteine-rich NH2-terminal domain that stabilizes the alpha-helix of the second collagen-like domain, and a third COOH-terminal carbohydrate binding region. The function of MBP is unknown, although a role in host defense is suggested by its ability to bind yeast mannans. In this report we show that native and recombinant human MBP can serve in an opsonic role in serum and thereby enhance clearance of mannose rich pathogens by phagocytes. MBP binds to wild-type virulent Salmonella montevideo that express a mannose-rich O-polysaccharide. Interaction of MBP with these organisms results in attachment, uptake, and killing of the opsonized bacteria by phagocytes. These results demonstrate that MBP plays a role in first line host defense against certain pathogenic organisms.

MeSH Terms
Antigens, Bacterial Carrier Proteins/physiology Fluorescent Antibody Technique Humans Mannose/metabolism Mannose-Binding Lectins Monocytes/physiology Mutation Neutrophils/physiology O Antigens Opsonin Proteins Oxygen Consumption Phagocytosis Polysaccharides, Bacterial/metabolism Recombinant Proteins Salmonella/genetics,metabolism
Chemicals
Antigens, Bacterial Carrier Proteins Mannose-Binding Lectins O Antigens Opsonin Proteins Polysaccharides, Bacterial Recombinant Proteins Mannose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kuhlman M
Harvard Department of Pediatrics, Children's Hospital, Boston, Massachusetts 02115.
Joiner K
Ezekowitz R A
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.169.5.1733
Published
1989-05-01
Pages
1733-45
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2189296
Subset
IM
Grants
NIAID NIH HHS · R01-AI-23786 · United States
External Links
PubMed source DOI: 10.1084/jem.169.5.1733 PMC Full Text View journal details
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