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PMID: 2475760 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Sequence and expression of human myosin alkali light chain isoforms.

Molecular and cellular biochemistry ·Vol. 87 ·No. 2 ·1989-06-01 ·Pages 119-36

Wade R, Feldman D, Gunning P, Kedes L

Abstract

In order to initiate the study of the functional differences between myosin alkali light chain isoforms and to investigate the mechanisms of their differential expression, we have isolated cDNA clones for two human alkali light chain isoforms. Here we report DNA sequence and RNA blotting analyses that demonstrate that these cDNAs represent transcripts encoding human MLC3F and MLC1Sb. The sequence of the human MLC1Sb cDNA offers the first fully characterized example of a slow-fiber skeletal muscle alkali light chain isoform from any species. The sequence analysis of these two cDNAs allows an examination of evolutionarily conserved features of mammalian alkali light chain genes. Examination of the genomic organization of the human alkali light chain isoform genes revealed that, in contrast with some strains of mice, both are single copy genes. RNA blot analysis conclusively demonstrates that the human skeletal muscle MLC1Sb gene is also expressed in the heart ventricle but not the atria. In addition, we examined the expression of alkali light chain isoforms during the in vitro differentiation of a variety of human and rodent myogenic cells and found striking variation in the pattern of alkali light chain isoform gene expression in different myogenic cells.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Biological Evolution Cell Line DNA/genetics DNA Probes Humans Isomerism Molecular Sequence Data Muscles/cytology,metabolism Myosin Subfragments Myosins/biosynthesis,genetics Nucleic Acid Hybridization Peptide Fragments/biosynthesis,genetics RNA/biosynthesis,genetics Restriction Mapping Rodentia/genetics Sequence Homology, Nucleic Acid
Chemicals
DNA Probes Myosin Subfragments Peptide Fragments RNA DNA Myosins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wade R
Department of Medicine, Stanford Medical School, Palo Alto, CA.
Feldman D
Gunning P
Kedes L
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42 references, click to expand
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Article Info
Journal
Molecular and cellular biochemistry
Abbr.
Mol Cell Biochem
ISSN
0300-8177
DOI
10.1007/BF00219255
Published
1989-06-01
Pages
119-36
Language
English
Region
Netherlands
NLM ID
0364456
Subset
IM
Grants
NCI NIH HHS · CA09302 · United States
External Links
PubMed source DOI: 10.1007/BF00219255 View journal details
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