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PMID: 2469000 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Selection and characterization of ricin toxin A-chain mutations in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 9 ·No. 2 ·1989-02-00 ·Pages 415-20

Frankel A, Schlossman D, Welsh P, Hertler A, Withers D, Johnston S

Abstract

A DNA sequence encoding the A chain of ricin toxin (RTA) from the castor bean plant, Ricinus communis, was placed under GAL1 promoter control and transformed into Saccharomyces cerevisiae. Induction of expression of RTA was lethal. This lethality was the basis for a selection of mutations in RTA which inactivated the toxin. A number of mutant alleles which encoded cross-reactive material were sequenced. Eight of the first nine mutant RTAs studied showed single-amino-acid changes involving residues located in the proposed active-site cleft.

MeSH Terms
Amino Acid Sequence Cloning, Molecular Codon/genetics DNA/genetics Genes, Lethal Immunochemistry Molecular Sequence Data Mutation Plant Lectins Plant Proteins/genetics Plants, Toxic Plasmids RNA/genetics Ricin/genetics,immunology Ricinus/genetics Saccharomyces cerevisiae/genetics
Chemicals
Codon Plant Lectins Plant Proteins RNA DNA Ricin
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Frankel A
Department of Medicine, Duke University, Durham, North Carolina 27710.
Schlossman D
Welsh P
Hertler A
Withers D
Johnston S
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.2.415-420.1989
Published
1989-02-00
Pages
415-20
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362616
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.9.2.415-420.1989 PMC Full Text View journal details
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