Home LiteratureArticle Details
PMID: 2468995 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Interaction of a hepatoma-specific nuclear factor with transcription-regulatory sequences of the human alpha-fetoprotein and albumin genes.

Molecular and cellular biology ·Vol. 8 ·No. 12 ·1988-12-00 ·Pages 5179-87

Sawadaishi K, Morinaga T, Tamaoki T

Abstract

DNase I footprinting and gel mobility shift analysis showed that an HuH-7 hepatoma nuclear protein, termed AFP1, binds specifically to an AT-rich sequence, TGATTAATAATTACA, in domain B of the human alpha-fetoprotein enhancer. No such binding activity was found in HeLa cell nuclei. Transient transfection studies showed that a 54-base-pair region corresponding to the AFP1-binding site could stimulate the simian virus 40 early promoter to express a linked chloramphenicol acetyltransferase gene in an orientation-independent and cell-specific manner. The correlation between the binding of AFP1 and the stimulation of chloramphenicol acetyltransferase gene expression strongly suggests that specific interaction of AFP1 with the AT motif is important for cell-specific transcriptional enhancement. Competition gel mobility shift analysis revealed that similar AT-rich sequences with high affinities to AFP1 were also present in the promoters of the alpha-fetoprotein and albumin genes. These results suggest that AFP1 may function as a common regulatory factor in the transcription of the alpha-fetoprotein and albumin genes.

MeSH Terms
Animals Base Sequence Carcinoma, Hepatocellular/genetics Cell Line DNA-Binding Proteins/metabolism Enhancer Elements, Genetic Genes Humans Liver Neoplasms/genetics Molecular Sequence Data Nuclear Proteins/metabolism Regulatory Sequences, Nucleic Acid Restriction Mapping Sequence Homology, Nucleic Acid Serum Albumin/genetics Transcription, Genetic alpha-Fetoproteins/genetics
Chemicals
DNA-Binding Proteins Nuclear Proteins Serum Albumin alpha-Fetoproteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sawadaishi K
Department of Medical Biochemistry, University of Calgary, Alberta, Canada.
Morinaga T
Tamaoki T
References (59)
59 references, click to expand
  1. Oncodevelopmental and hormonal regulation of alpha 1-fetoprotein gene expression.
    Adv Enzyme Regul. 1983;21:73-99 PMID: 6085825
  2. Cell type-specific transcriptional enhancement in vitro requires the presence of trans-acting factors.
    EMBO J. 1985 Nov;4(11):3005-13 PMID: 2998773
  3. The human albumin gene. Characterization of the 5' and 3' flanking regions and the polymorphic gene transcripts.
    J Biol Chem. 1986 Mar 5;261(7):3244-51 PMID: 2419329
  4. Regulated expression of alpha-fetoprotein genes in transgenic mice.
    Cold Spring Harb Symp Quant Biol. 1985;50:371-8 PMID: 2420515
  5. Molecular structure of the human albumin gene is revealed by nucleotide sequence within q11-22 of chromosome 4.
    J Biol Chem. 1986 May 25;261(15):6747-57 PMID: 3009475
  6. Detection of factors that interact with the human beta-interferon regulatory region in vivo by DNAase I footprinting.
    Cell. 1986 May 23;45(4):611-8 PMID: 3708689
  7. Tandem kappa immunoglobulin promoters are equally active in the presence of the kappa enhancer: implications for models of enhancer function.
    Cell. 1986 Jul 18;46(2):253-62 PMID: 3087628
  8. Synergism between immunoglobulin enhancers and promoters.
    Nature. 1986 Jul 24-30;322(6077):383-5 PMID: 3016550
  9. Gene regulation by proteins acting nearby and at a distance.
    Nature. 1986 Aug 21-27;322(6081):697-701 PMID: 3018583
  10. Transcriptional selectivity of viral genes in mammalian cells.
    Cell. 1986 Sep 12;46(6):795-805 PMID: 3530495
  11. Cell type-specific negative regulatory element in the control region of the rat alpha-fetoprotein gene.
    Proc Natl Acad Sci U S A. 1986 Oct;83(20):7653-7 PMID: 2429314
  12. Liver-specific expression of the mouse alpha-fetoprotein gene is mediated by cis-acting DNA elements.
    Proc Natl Acad Sci U S A. 1986 Nov;83(21):8196-200 PMID: 2430280
  13. Tissue-specific in vitro transcription from the mouse albumin promoter.
    Cell. 1986 Dec 5;47(5):767-76 PMID: 3779841
  14. Inducibility of kappa immunoglobulin enhancer-binding protein Nf-kappa B by a posttranslational mechanism.
    Cell. 1986 Dec 26;47(6):921-8 PMID: 3096580
  15. Multiple regulatory elements in the intergenic region between the alpha-fetoprotein and albumin genes.
    Mol Cell Biol. 1986 Feb;6(2):477-87 PMID: 2431269
  16. The role of the kappa enhancer and its binding factor NF-kappa B in the developmental regulation of kappa gene transcription.
    Cell. 1987 Jan 16;48(1):121-8 PMID: 3098435
  17. Diversity of alpha-fetoprotein gene expression in mice is generated by a combination of separate enhancer elements.
    Science. 1987 Jan 2;235(4784):53-8 PMID: 2432657
  18. Cell-specific enhancer activity in a far upstream region of the human alpha-fetoprotein gene.
    J Biol Chem. 1987 Apr 5;262(10):4812-8 PMID: 2435718
  19. Raf, a trans-acting locus, regulates the alpha-fetoprotein gene in a cell-autonomous manner.
    Science. 1987 Apr 17;236(4799):301-3 PMID: 2436297
  20. Purified transcription factor AP-1 interacts with TPA-inducible enhancer elements.
    Cell. 1987 Jun 19;49(6):741-52 PMID: 3034433
  21. Regulation of inducible and tissue-specific gene expression.
    Science. 1987 Jun 5;236(4806):1237-45 PMID: 3296191
  22. Factors involved in control of tissue-specific expression of albumin gene.
    Cell. 1987 Aug 14;50(4):627-38 PMID: 3607880
  23. The rat alpha-fetoprotein and albumin genes. Transcriptional control and comparison of the sequence organization and promoter region.
    J Biol Chem. 1987 Sep 15;262(26):12479-87 PMID: 2442163
  24. Multiple nuclear proteins in liver cells are bound to hepatitis B virus enhancer element and its upstream sequences.
    EMBO J. 1987 Jul;6(7):1913-20 PMID: 3653073
  25. Tissue-specific expression of the human alpha 1-antitrypsin gene is controlled by multiple cis-regulatory elements.
    Nucleic Acids Res. 1987 Oct 26;15(20):8399-415 PMID: 2823229
  26. Interaction of a liver-specific nuclear factor with the fibrinogen and alpha 1-antitrypsin promoters.
    Science. 1987 Oct 30;238(4827):688-92 PMID: 3499668
  27. The regulation of albumin and alpha-fetoprotein gene expression in mammals.
    Biochimie. 1987 May;69(5):445-59 PMID: 2445387
  28. Cis- and trans-acting elements responsible for the cell-specific expression of the human alpha 1-antitrypsin gene.
    EMBO J. 1987 Sep;6(9):2759-66 PMID: 2824193
  29. An albumin enhancer located 10 kb upstream functions along with its promoter to direct efficient, liver-specific expression in transgenic mice.
    Genes Dev. 1987 May;1(3):268-76 PMID: 3678824
  30. The interplay of DNA-binding proteins on the promoter of the mouse albumin gene.
    Cell. 1987 Dec 24;51(6):963-73 PMID: 3690666
  31. The cell-specific enhancer of the mouse transthyretin (prealbumin) gene binds a common factor at one site and a liver-specific factor(s) at two other sites.
    Mol Cell Biol. 1988 Jan;8(1):81-90 PMID: 3336368
  32. Activation of cell-specific expression of rat growth hormone and prolactin genes by a common transcription factor.
    Science. 1988 Mar 18;239(4846):1400-5 PMID: 2831625
  33. Fine-structure mapping of the three mouse alpha-fetoprotein gene enhancers.
    Mol Cell Biol. 1988 Mar;8(3):1169-78 PMID: 2452972
  34. Enhancer and promoter elements directing activation and glucocorticoid repression of the alpha 1-fetoprotein gene in hepatocytes.
    Mol Cell Biol. 1988 Apr;8(4):1398-407 PMID: 2454390
  35. The structure and regulation of the alpha-fetoprotein and albumin genes.
    Oxf Surv Eukaryot Genes. 1985;2:160-206 PMID: 2474300
  36. A new technique for the assay of infectivity of human adenovirus 5 DNA.
    Virology. 1973 Apr;52(2):456-67 PMID: 4705382
  37. A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the Escherichia coli lactose operon regulatory system.
    Nucleic Acids Res. 1981 Jul 10;9(13):3047-60 PMID: 6269071
  38. Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.
    Nucleic Acids Res. 1981 Dec 11;9(23):6505-25 PMID: 6275366
  39. Transcriptional control of the murine albumin/alpha-fetoprotein locus during development.
    Proc Natl Acad Sci U S A. 1982 Sep;79(17):5254-7 PMID: 6182563
  40. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
    Mol Cell Biol. 1982 Sep;2(9):1044-51 PMID: 6960240
  41. The Rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA-mediated transfection.
    Proc Natl Acad Sci U S A. 1982 Nov;79(22):6777-81 PMID: 6294651
  42. Genetic analysis of alpha-fetoprotein synthesis in mice.
    Mol Cell Biol. 1982 Nov;2(11):1427-35 PMID: 6186903
  43. Transient expression of a mouse alpha-fetoprotein minigene: deletion analyses of promoter function.
    Mol Cell Biol. 1983 Jul;3(7):1295-309 PMID: 6193415
  44. Two protein-binding sites in chromatin implicated in the activation of heat-shock genes.
    Nature. 1984 May 17-23;309(5965):229-34 PMID: 6325944
  45. Potential basis for regulation of the coordinately expressed fibrinogen genes: homology in the 5' flanking regions.
    Proc Natl Acad Sci U S A. 1984 Apr;81(8):2313-6 PMID: 6232608
  46. Structure of the 5' ends of immunoglobulin genes: a novel conserved sequence.
    Proc Natl Acad Sci U S A. 1984 May;81(9):2650-4 PMID: 6425835
  47. Phenotypical stability of a human hepatoma cell line, HuH-7, in long-term culture with chemically defined medium.
    Gan. 1984 Feb;75(2):151-8 PMID: 6203805
  48. Correct transcription of an immunoglobulin kappa gene requires an upstream fragment containing conserved sequence elements.
    Nature. 1984 Jul 5-11;310(5972):71-4 PMID: 6330567
  49. A protein binds to a satellite DNA repeat at three specific sites that would be brought into mutual proximity by DNA folding in the nucleosome.
    Cell. 1984 Jul;37(3):889-901 PMID: 6540146
  50. Tissue-specific activation of a cloned alpha-fetoprotein gene during differentiation of a transfected embryonal carcinoma cell line.
    Nature. 1984 Aug 16-22;310(5978):562-7 PMID: 6205280
  51. Complete sequence of the cDNA for human alpha 1-antitrypsin and the gene for the S variant.
    Biochemistry. 1984 Oct 9;23(21):4828-37 PMID: 6093867
  52. Tissue-specific expression is conferred by a sequence from the 5' end of the rat albumin gene.
    EMBO J. 1984 Nov;3(11):2505-10 PMID: 6096126
  53. Cell-type-specific contacts to immunoglobulin enhancers in nuclei.
    Nature. 1985 Feb 28-Mar 6;313(6005):798-801 PMID: 3919308
  54. Transcription cell type specificity is conferred by an immunoglobulin VH gene promoter that includes a functional consensus sequence.
    Cell. 1985 Jun;41(2):479-87 PMID: 3921262
  55. The human alpha-fetoprotein gene. Sequence organization and the 5' flanking region.
    J Biol Chem. 1985 Apr 25;260(8):5055-60 PMID: 2580830
  56. Cell-type specificity of immunoglobulin gene expression is regulated by at least three DNA sequence elements.
    Cell. 1985 Jul;41(3):885-97 PMID: 3924411
  57. Developmental regulation of alpha-fetoprotein genes in transgenic mice.
    Mol Cell Biol. 1985 Jul;5(7):1639-48 PMID: 2410773
  58. An exonuclease protection assay reveals heat-shock element and TATA box DNA-binding proteins in crude nuclear extracts.
    Nature. 1985 Sep 5-11;317(6032):84-7 PMID: 2993917
  59. A nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genes.
    Nature. 1986 Jan 9-15;319(6049):154-8 PMID: 3079885
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.12.5179-5187.1988
Published
1988-12-00
Pages
5179-87
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365620
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.8.12.5179-5187.1988 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2