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PMID: 2468648 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Role of exonuclease III and endonuclease IV in repair of pyrimidine dimers initiated by bacteriophage T4 pyrimidine dimer-DNA glycosylase.

Journal of bacteriology ·Vol. 171 ·No. 5 ·1989-05-00 ·Pages 2542-6

Saporito SM, Gedenk M, Cunningham RP

Abstract

The role of exonuclease III and endonuclease IV in the repair of pyrimidine dimers in bacteriophage T4-infected Escherichia coli was examined. UV-irradiated T4 showed reduced survival when plated on an xth nfo double mutant but showed wild-type survival on either single mutant. T4 denV phage were equally sensitive when plated on wild-type E. coli or an xth nfo double mutant, suggesting that these endonucleases function in the same repair pathway as T4 pyrimidine dimer-DNA glycosylase. A uvrA mutant of E. coli in which the repair of pyrimidine dimers was dependent on the T4 denV gene carried on a plasmid was constructed. Neither an xth nor an nfo derivative of this strain was more sensitive than the parental strain to UV irradiation. We were unable to construct a uvrA xth nfo triple mutant. In addition, T4, which turns off the host UvrABC excision nuclease, showed reduced plating efficiency on an xth nfo double mutant.

MeSH Terms
Apurinic Acid/metabolism DNA Glycosylases DNA Repair DNA-(Apurinic or Apyrimidinic Site) Lyase Deoxyribonuclease IV (Phage T4-Induced) Endodeoxyribonucleases/physiology Escherichia coli/genetics Escherichia coli Proteins Exodeoxyribonucleases/physiology Mutation N-Glycosyl Hydrolases/metabolism Polynucleotides/metabolism Pyrimidine Dimers/metabolism T-Phages/enzymology,genetics,radiation effects Ultraviolet Rays Virus Replication
Chemicals
Escherichia coli Proteins Polynucleotides Pyrimidine Dimers Apurinic Acid Endodeoxyribonucleases Exodeoxyribonucleases exodeoxyribonuclease III Deoxyribonuclease IV (Phage T4-Induced) endonuclease IV, E coli DNA Glycosylases N-Glycosyl Hydrolases deoxyribopyrimidine endonucleosidase DNA-(Apurinic or Apyrimidinic Site) Lyase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Saporito S M
Department of Biological Sciences, State University of New York, Albany 12222.
Gedenk M
Cunningham R P
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32 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.5.2542-2546.1989
Published
1989-05-00
Pages
2542-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC209932
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.171.5.2542-2546.1989 PMC Full Text View journal details
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